ELISA Kit for Bovine Pepsin A

Mfr.No.

E0632b

Categories

Elisa

Function

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Specificity

Natural and recombinant Bovine Pepsin A

Subcellular Location

Secreted

Entryname

PEPA_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pepsin A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-98%	78-92%	86-98%	100-106%
EDTA plasma(n=5)	83-92%	86-99%	91-104%	87-98%
heparin plasma(n=5)	86-101%	89-97%	93-105%	90-102%

Platform

PF07966

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

Protein Name

Pepsin A

Recovery

Recovery was determined by spiking various levels of Pepsin A into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	97	94-99
Plasma	96	92-102

Sequence

50:

MSVVKIPLVK ; KKSLRQNLIE ; NGKLKEFMRT ; HKYNLGSKYI ; REAATLVSEQ ;

100:

PLQNYLDTEY ; FGTIGIGTPA ; QDFTVIFDTG ; SSNLWVPSIY ; CSSEACTNHN ;

150:

RFNPQDSSTY ; EATSETLSIT ; YGTGSMTGIL ; GYDTVQVGGI ; SDTNQIFGLS ;

200:

ETEPGSFLYY ; APFDGILGLA ; YPSISSSGAT ; PVFDNIWDQG ; LVSQDLFSVY ;

250:

LSSNEESGSV ; VIFGDIDSSY ; YSGSLNWVPV ; SVEGYWQITV ; DSITMNGESI ;

300:

ACSDGCQAIV ; DTGTSLLAGP ; TTAISNIQSY ; IGASEDSSGE ; VVISCSSIDS ;

350:

LPDIVFTING ; VQYPVPPSAY ; ILQSNGICSS ; GFEGMDISTS ; SGDLWILGDV ;

372:

FIRQYFTVFD ; RGNNQIGLAP ; VA

Sequence Length

372

String

P00792

Uniprot	P00792
Gene Name	PGA
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:414350
Unigene	Bt.15850

Reference	1. "Cloning of the authentic bovine gene encoding pepsinogen A and its expression in microbial cells." Munoz R., Garcia J.L., Carrascosa A.V., Gonzalez R. Appl. Environ. Microbiol.70:2588-2595(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "The activation of bovine pepsinogen. Sequence of the peptides released, identification of a pepsin inhibitor." Harboe M.K., Andersen P.M., Foltmann B., Kay J., Kassell B. J. Biol. Chem.249:4487-4494(1974) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 2-48 3. "Bovine pepsin: the sequence of the first 65 amino acid residues (completing the sequence of the first 110 residues of bovine pepsinogen)." Harboe M.K., Foltmann B. FEBS Lett.60:133-136(1975) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 42-111

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808454	E0632b	96T	$900		Add to Cart

ELISA Kit for Bovine Pepsin A

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