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ELISA Kit for Bovine Nitric oxide synthase, endothelial

SKU : BHE13808233

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E0815b
Alternative Names	Constitutive NOS ; cNOS ; EC-NOS ; Endothelial NOS ; eNOS ; NOS type III ; NOSIII
Categories	Elisa
Function	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Specificity	Natural and recombinant Bovine Nitric oxide synthase, endothelial
Subcellular Location	Cell membrane Membrane caveola Cytoplasm cytoskeleton Golgi apparatus Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Detection Range	0.156-10ng/mL
Entryname	NOS3_BOVIN
Method Type	Sandwich
Platform	PF00667
Protein Name	Nitric oxide synthase, endothelial
Sensitivity	0.067ng/mL
Sequence	50: MGNLKSVGQE ; PGPPCGLGLG ; LGLGLCGKQG ; PASPAPEPSR ; APAPATPHAP ; 100: DHSPAPNSPT ; LTRPPEGPKF ; PRVKNWELGS ; ITYDTLCAQS ; QQDGPCTPRC ; 150: CLGSLVLPRK ; LQTRPSPGPP ; PAEQLLSQAR ; DFINQYYSSI ; KRSGSQAHEE ; 200: RLQEVEAEVA ; STGTYHLRES ; ELVFGAKQAW ; RNAPRCVGRI ; QWGKLQVFDA ; 250: RDCSSAQEMF ; TYICNHIKYA ; TNRGNLRSAI ; TVFPQRAPGR ; GDFRIWNSQL ; 300: VRYAGYRQQD ; GSVRGDPANV ; EITELCIQHG ; WTPGNGRFDV ; LPLLLQAPDE ; 350: APELFVLPPE ; LVLEVPLEHP ; TLEWFAALGL ; RWYALPAVSN ; MLLEIGGLEF ; 400: SAAPFSGWYM ; STEIGTRNLC ; DPHRYNILED ; VAVCMDLDTR ; TTSSLWKDKA ; 450: AVEINLAVLH ; SFQLAKVTIV ; DHHAATVSFM ; KHLDNEQKAR ; GGCPADWAWI ; 500: VPPISGSLTP ; VFHQEMVNYI ; LSPAFRYQPD ; PWKGSATKGA ; GITRKKTFKE ; 550: VANAVKISAS ; LMGTLMAKRV ; KATILYASET ; GRAQSYAQQL ; GRLFRKAFDP ; 600: RVLCMDEYDV ; VSLEHEALVL ; VVTSTFGNGD ; PPENGESFAA ; ALMEMSGPYN ; 650: SSPRPEQHKS ; YKIRFNSVSC ; SDPLVSSWRR ; KRKESSNTDS ; AGALGTLRFC ; 700: VFGLGSRAYP ; HFCAFARAVD ; TRLEELGGER ; LLQLGQGDEL ; CGQEEAFRGW ; 750: AKAAFQASCE ; TFCVGEEAKA ; AAQDIFSPKR ; SWKRQRYRLS ; TQAEGLQLLP ; 800: GLIHVHRRKM ; FQATVLSVEN ; LQSSKSTRAT ; ILVRLDTAGQ ; EGLQYQPGDH ; 850: IGICPPNRPG ; LVEALLSRVE ; DPPPPTESVA ; VEQLEKGSPG ; GPPPSWVRDP ; 900: RLPPCTLRQA ; LTFFLDITSP ; PSPRLLRLLS ; TLAEEPSEQQ ; ELETLSQDPR ; 950: RYEEWKWFRC ; PTLLEVLEQF ; PSVALPAPLL ; LTQLPLLQPR ; YYSVSSAPNA ; 1000: HPGEVHLTVA ; VLAYRTQDGL ; GPLHYGVCST ; WLSQLKTGDP ; VPCFIRGAPS ; 1050: FRLPPDPYVP ; CILVGPGTGI ; APFRGFWQER ; LHDIESKGLQ ; PAPMTLVFGC ; 1100: RCSQLDHLYR ; DEVQDAQERG ; VFGRVLTAFS ; REPDSPKTYV ; QDILRTELAA ; 1150: EVHRVLCLER ; GHMFVCGDVT ; MATSVLQTVQ ; RILATEGDME ; LDEAGDVIGV ; 1200: LRDQQRYHED ; IFGLTLRTQE ; VTSRIRTQSF ; SLQERHLRGA ; VPWAFDPPGP ; 1205: DTPGP
Sequence Length	1205
String	P29473
Sub Unit	Homodimer. Interacts with NOSIP and NOSTRIN.

Uniprot	P29473
Gene Name	NOS3
Immunogen Species	Bos Taurus (Cattle)
Unigene	Bt.4662

Reference	1. "Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform." Lamas S., Marsden P.A., Li G.K., Tempst P., Michel T. Proc. Natl. Acad. Sci. U.S.A.89:6348-6352(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "Molecular cloning and characterization of the constitutive bovine aortic endothelial cell nitric oxide synthase." Nishida K., Harrison D.G., Navas J.P., Fisher A.A., Dockery S.P., Uematsu M., Nerem R.M., Alexander R.W., Murphy T.J. J. Clin. Invest.90:2092-2096(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 3. "Molecular cloning and expression of a cDNA encoding endothelial cell nitric oxide synthase." Sessa W.C., Harrison J.K., Barber C.M., Zeng D., Durieux M.E., D'Angelo D.D., Lynch K.R., Peach M.J. J. Biol. Chem.267:15274-15276(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Aortic endothelium. 4. "Endothelial nitric oxide synthase. N-terminal myristoylation determines subcellular localization." Busconi L., Michel T. J. Biol. Chem.268:8410-8413(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MYRISTOYLATION AT GLY-2 5. "Mutagenesis of palmitoylation sites in endothelial nitric oxide synthase identifies a novel motif for dual acylation and subcellular targeting." Robinson L.J., Michel T. Proc. Natl. Acad. Sci. U.S.A.92:11776-11780(1995) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PALMITOYLATION AT CYS-15 AND CYS-26 6. "Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism." Boo Y.C., Hwang J., Sykes M., Michell B.J., Kemp B.E., Lum H., Jo H. Am. J. Physiol.283:H1819-H1828(2002) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-497; SER-635 AND SER-1179 7. "Aberrant cytoplasmic sequestration of eNOS in endothelial cells after monocrotaline, hypoxia, and senescence: live-cell caveolar and cytoplasmic NO imaging." Mukhopadhyay S., Xu F., Sehgal P.B. Am. J. Physiol.292:H1373-H1389(2007) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION 8. "Crystal structure of constitutive endothelial nitric oxide synthase: a paradigm for pterin function involving a novel metal center." Raman C.S., Li H., Martasek P., Kral V., Masters B.S.S., Poulos T.L. Cell95:939-950(1998) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 67-482 9. "Mapping the active site polarity in structures of endothelial nitric oxide synthase heme domain complexed with isothioureas." Li H., Raman C.S., Martasek P., Kral V., Masters B.S.S., Poulos T.L. J. Inorg. Biochem.81:133-139(2000) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) OF 67-482 10. "Crystallographic studies on endothelial nitric oxide synthase complexed with nitric oxide and mechanism-based inhibitors." Li H., Raman C.S., Martasek P., Masters B.S.S., Poulos T.L. Biochemistry40:5399-5406(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) 11. "Crystal structure of nitric oxide synthase bound to nitro indazole reveals a novel inactivation mechanism." Raman C.S., Li H., Martasek P., Southan G., Masters B.S.S., Poulos T.L. Biochemistry40:13448-13455(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) 12. "Implications for isoform-selective inhibitor design derived from the binding mode of bulky isothioureas to the heme domain of endothelial nitric-oxide synthase." Raman C.S., Li H., Martasek P., Babu B.R., Griffith O.W., Southan G., Masters B.S.S., Poulos T.L. J. Biol. Chem.276:26486-26491(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) 13. "Structural basis for pterin antagonism in nitric-oxide synthase. Development of novel 4-oxo-pteridine antagonists of (6R)-5,6,7,8-tetrahydrobiopterin." Kotsonis P., Frohlich L.G., Raman C.S., Li H., Berg M., Gerwig R., Groehn V., Kang Y., Al-Masoudi N., Taghavi-Moghadam S., Mohr D., Munch U., Schnabel J., Martasek P., Masters B.S.S., Strobel H., Poulos T., Matter H., Pfleiderer W., Schmidt H.H.H.W.more... J. Biol. Chem.276:49133-49141(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS)

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