ELISA Kit for Bovine Interleukin-18

Mfr.No.

E0064b

Alternative Names

Interferon gamma-inducing factor ; IFN-gamma-inducing factor ; Interleukin-1 gamma ; IL-1 gamma ; IGIF

Categories

Elisa

Function

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

Specificity

Natural and recombinant Bovine Interleukin-18

Subcellular Location

Secreted

Detection Range

15.6-1000pg/mL

Entryname

IL18_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	100-105%	94-101%	95-102%	92-97%
EDTA plasma(n=5)	90-95%	94-106%	105-107%	87-98%
heparin plasma(n=5)	95-103%	91-99%	104-107%	98-105%

Method Type

Sandwich

Platform

PF00340

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Protein Name

Interleukin-18

Recovery

Recovery was determined by spiking various levels of IL-18 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	102	96-106
Plasma	101	99-104

Sensitivity

7pg/mL

Sequence

50:

MAAEQVEDYC ; ISFVEMKFIN ; NTLYFVAEND ; EDLESDHFGK ; LEPKLSIIRN ;

100:

LNDQVLFINQ ; GNQPVFEDMP ; DSDCSDNAPQ ; TIFIIYMYKD ; SLTRGLAVTI ;

150:

SVQCKKMSTL ; SCENKIVSFK ; EMNPPDNIDN ; EESDIIFFQR ; SVPGHDDKIQ ;

193:

FESSLYKGYF ; LACKKENDLF ; KLILKKQDDN ; RDKSVMFTVQ ; NQN

Sequence Length

193

String

Q9TU73

Uniprot	Q9TU73
Gene Name	IL18
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:281249
Unigene	Bt.234

Reference	1. "Cloning of a cDNA encoding bovine interleukin-18 and analysis of IL-18 expression in macrophages and its IFN-gamma-inducing activity." Shoda L.K., Zarlenga D.S., Hirano A., Brown W.C. J. Interferon Cytokine Res.19:1169-1177(1999) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "U.S. veterinary immune reagent network: expressed bovine gene sequences." U.S. Veterinary Immune Reagent Network, Hudgens T., Tompkins D., Baldwin C.L. Submitted (2007-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Belted Galloway. tissue: Peripheral blood. 3. NIH - Mammalian Gene Collection (MGC) project Submitted (2005-08) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Crossbred X Angus. tissue: Ileum.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805688	E0064b	96T	$900		Add to Cart

ELISA Kit for Bovine Interleukin-18

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