ELISA Kit for Bovine Interleukin-17A

Mfr.No.

E0063b

Categories

Elisa

Function

Induces stromal cells to produce proinflammatory and hematopoietic cytokines.

Specificity

Natural and recombinant Bovine Interleukin-17A

Subcellular Location

Secreted

Detection Range

78-5000pg/mL

Entryname

IL17_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL17A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	97-105%	95-103%	95-106%	98-109%
EDTA plasma(n=5)	99-105%	97-105%	99-110%	97-106%
heparin plasma(n=5)	95-104%	99-110%	100-111%	99-110%

Method Type

Sandwich

Platform

PF06083

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.2%

Protein Name

Interleukin-17A

Recovery

Recovery was determined by spiking various levels of IL17A into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	97-106
Plasma	103	96-105

Sensitivity

32pg/mL

Sequence

50:

MASMRTSSMS ; LLLLLSLVAL ; VKAGVIIPQS ; PGCPPTEDKN ; FPQHVRVNLN ;

100:

IVNRSTNSRR ; PTDYHKRSTS ; PWTLHRNEDP ; ERYPSVIWEA ; KCSHSGCINA ;

150:

EGKVDHHMNS ; VTIQQEILVL ; RRESQHCPHS ; FRLEKMLVAV ; GCTCVTPIVR ;

153:

HLA

Sequence Length

153

String

Q687Y7

Sub Unit

Homodimer.

Uniprot	Q687Y7
Gene Name	IL17A
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:282863
Unigene	Bt.12772

Reference	1. "Neutrophil recruitment in the bovine mammary gland." Riollet C., Rainard P. Submitted (2001-08) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. Lee I.-K., Mwangi S.M., Olsen S., Kehrli M. Jr. Submitted (2001-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-153

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805668	E0063b	96T	$900		Add to Cart

ELISA Kit for Bovine Interleukin-17A

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