ELISA Kit for Bovine Interleukin-1 receptor-associated kinase 1

Mfr.No.

E1514b

Categories

Elisa

Function

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-TAK1/NR2C2-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, TAK1/NR2C2 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Specificity

Natural and recombinant Bovine Interleukin-1 receptor-associated kinase 1

Subcellular Location

Cytoplasm Nucleus Translocates to the nucleus when sumoylated.

Entryname

IRAK1_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IRAK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-110%	102-110%	101-109%	99-109%
EDTA plasma(n=5)	98-113%	99-107%	96-112%	97-108%
heparin plasma(n=5)	100-107%	97-110%	102-107%	100-106%

Platform

PF00531

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.3%

Protein Name

Interleukin-1 receptor-associated kinase 1

Recovery

Recovery was determined by spiking various levels of IRAK1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	100-110
Plasma	104	102-112

Sequence

50:

MAGGPGPGDP ; AVPGAQHFLY ; EVPPWVMCRF ; YKVMDALEPA ; DWCQFAALIV ;

100:

RDQTELRLCE ; RSGQRTASVL ; WPWINRNARV ; ADLVRILTHL ; QLLRARDIIT ;

150:

AWHPPAPLLP ; PSTTSLTPSS ; ISAPSEAAVP ; GHRKLPSLAS ; TFLSPAFPGS ;

200:

QTHSDPELCP ; GPSPAAHQPP ; LPSPAPSSTK ; PSPESPMSLL ; PGAPSSSFCW ;

250:

PLHEICQGTH ; DFSEELKIGE ; GGFGCVYRAV ; MRNTVYAVKR ; LKEEADLEWT ;

300:

TVKQSFQTEV ; QQLSRFRHPN ; IVDFAGYCAQ ; SGFYCLVYGF ; LPNGSLEDRL ;

350:

HVQTQAWPPL ; SWPQRLDILL ; GTARAIQFLH ; QDSPSLIHGD ; VKSSNVLLDE ;

400:

RLMPKLGDFG ; LARLSRFTGA ; NPGQSSSVAR ; TRTVRGTLAY ; LPEEYVKTGR ;

450:

LAVDTDTFSF ; GVVLLETLAG ; QRAVRMHGAQ ; PKYLKDLVEE ; EAEEAGVTLK ;

500:

GTQTAVQGGP ; AADTWAALVA ; AQIYKKHLDP ; RPGPCPPQLG ; LALGQLACCC ;

550:

LHRRAKRRPP ; MTQVYQTLEE ; LQVVVAGPCL ; ELEAASRSPP ; SPQENSYVST ;

600:

SGSALSRASP ; WQPLAAPLGA ; QAQATDWPQK ; GANQPVESDE ; SVSDLSAALH ;

650:

SWHLSPSCPA ; GPGAPSWVPA ; PFGQAACTQG ; GAARESSCGS ; GPGLQPTAVE ;

700:

GPLLGSSMSS ; RPPQIVINPA ; RRKMLQKLAL ; YEDGVLDSLQ ; LLSSSSLPDS ;

718:

GQDLQDRQGP ; EERDEFRS

Sequence Length

718

String

Q2LGB3

Sub Unit

Homodimer (By similarity). Interacts with TOLLIP; this interaction occurs in the cytosol prior to receptor activation. Interacts with MYD88; this interaction recruits IRAK1 to the stimulated receptor complex. Interacts with IL1RL1. Interacts with IRAK1BP1 (By similarity). Associates with TRAF6, PELI1 and IRAK4; this complex recruits TAK1/NR2C2, TAB1 and TAB2 to mediate NF-kappa-B activation. Interacts (when polyubiquitinated) with IKBKG/NEMO.

Uniprot	Q2LGB3
Gene Name	IRAK1
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:533953
Unigene	Bt.5221

Reference	1. "Cloning and radiation hybrid mapping of bovine toll-like receptor-4 (TLR-4) signaling molecules." Connor E.E., Cates E.A., Williams J.L., Bannerman D.D. Vet. Immunol. Immunopathol.112:302-308(2006) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Mammary gland.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13806248	E1514b	96T	$900		Add to Cart

ELISA Kit for Bovine Interleukin-1 receptor-associated kinase 1

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