ELISA Kit for Bovine Inhibin beta A chain

Mfr.No.

E0838b

Alternative Names

Activin beta-A chain

Categories

Elisa

Function

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Specificity

Natural and recombinant Bovine Inhibin beta A chain

Subcellular Location

Secreted

Detection Range

78-5000pg/mL

Entryname

INHBA_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-104%	93-101%	95-105%	91-108%
EDTA plasma(n=5)	89-96%	93-106%	92-97%	94-106%
heparin plasma(n=5)	93-105%	94-108%	101-111%	92-109%

Method Type

Sandwich

Platform

PF00019

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 7.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.7%

Protein Name

Inhibin beta A chain

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	101	95-107

Sensitivity

37pg/mL

Sequence

50:

MPLLWLRGFL ; LASCWIIVRS ; SPTPGSEGHS ; AAPDCPSCAL ; ATLPKDVPNS ;

100:

QPEMVEAVKK ; HILNMLHLKK ; RPDVTQPVPK ; AALLNAIRKL ; HVGKVGENGY ;

150:

VEIEDDIGRR ; AEMNELMEQT ; SEIITFAESG ; TARKTLHFEI ; SKEGSDLSVV ;

200:

ERAEIWLFLK ; VPKANRTRSK ; VTIRLFQQQK ; HLQGSLDAGE ; EAEEVGLKGE ;

250:

KSEMLISEKV ; VDARKSTWHI ; FPVSSCIQRL ; LDQGKSSLDI ; RIACEQCQET ;

300:

GASLVLLGKK ; KKKEEEGEGK ; KRDGEGGAGG ; DEEKEQSHRP ; FLMLQARQSE ;

350:

DHPHRRRRRG ; LECDGKVNIC ; CKKQFFVSFK ; DIGWNDWIIA ; PSGYHANYCE ;

400:

GECPSHIAGT ; SGSSLSFHST ; VINHYRMRGH ; SPFANLKSCC ; VPTKLRPMSM ;

425:

LYYDDGQNII ; KKDIQNMIVE ; ECGCS

Sequence Length

425

String

P07995

Sub Unit

Dimeric, linked by one or more disulfide bonds. Inhibin A is a dimer of alpha and beta-A. Inhibin B is a dimer of alpha and beta-B. Activin A is a homodimer of beta-A. Activin B is a homodimer of beta-B. Activin AB is a dimer of beta-A and beta-B. Interacts with FST and FSTL3.

Uniprot	P07995
Gene Name	INHBA
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:281867
Unigene	Bt.12760

Reference	1. "Genomic cloning and sequence analyses of the bovine alpha-, beta A- and beta B-inhibin/activin genes. Identification of transcription factor AP-2-binding sites in the 5'-flanking regions by DNase I footprinting." Thompson D.A., Cronin C.N., Martin F. Eur. J. Biochem.226:751-764(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] tissue: Liver. 2. NIH - Mammalian Gene Collection (MGC) project Submitted (2006-10) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Hereford. tissue: Fetal brain. 3. "Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid." Forage R.G., Ring J.M., Brown R.W., McInerney B.V., Cobon G.S., Gregson R.P., Robertson D.M., Morgan F.J., Hearn M.T.W., Findlay J.K., Wettenhall R.E.H., Burger H.G., de Kretser D.M. Proc. Natl. Acad. Sci. U.S.A.83:3091-3095(1986) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 258-425 tissue: Ovarian follicular fluid. 4. "Purification and characterization of high molecular weight forms of inhibin from bovine follicular fluid." Sugino K., Nakamura T., Takio K., Miyamoto K., Hasegawa Y., Igarashi M., Titani K., Sugino H. Endocrinology130:789-796(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE

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ELISA Kit for Bovine Inhibin beta A chain

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