ELISA Kit for Bovine Glutathione peroxidase 3

Mfr.No.

E9949b

Alternative Names

Plasma glutathione peroxidase ; GPx-P

Categories

Elisa

Function

Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Specificity

Natural and recombinant Bovine Glutathione peroxidase 3

Subcellular Location

Secreted

Detection Range

0.78-50ng/ml

Entryname

GPX3_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-101%	92-103%	92-105%	94-106%
EDTA plasma(n=5)	89-97%	93-102%	92-98%	90-103%
heparin plasma(n=5)	93-101%	94-104%	101-108%	91-104%

Method Type

Competitive

Platform

PF00255

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Protein Name

Glutathione peroxidase 3

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-104
Plasma	95	92-103

Sensitivity

0.24ng/ml

Sequence

50:

MARLFRASCL ; LSLLLAGFIP ; PSQGQEKSKT ; DCHAGVGGTI ; YEYGALTIDG ;

100:

EEYIPFKQYA ; GKYILFVNVA ; SYUGLTGQYV ; ELNALQEELE ; PFGLVILGFP ;

150:

CNQFGKQEPG ; ENSEILATLK ; YVRPGGGFTP ; NFQLFEKGDV ; NGEKEQKFYT ;

200:

FLKNSCPPTS ; ELLGSPDRLF ; WEPMKVHDIR ; WNFEKFLVGP ; DGIPIMRWYH ;

226:

RTTVNSVKMD ; ILTYMRRRAV ; WEAKGK

Sequence Length

226

String

P37141

Sub Unit

Homotetramer.

Uniprot	P37141
Gene Name	GPX3
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:281210
Unigene	Bt.12916

Reference	1. "Cloning of the bovine plasma selenium-dependent glutathione peroxidase (GP) cDNA from the ocular ciliary epithelium: expression of the plasma and cellular forms within the mammalian eye." Martin-Alonso J.M., Ghosh S., Coca-Prados M. J. Biochem.114:284-291(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Eye. 2. NIH - Mammalian Gene Collection (MGC) project Submitted (2007-07) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Hereford. tissue: Testis.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805105	E9949b	96T	$900		Add to Cart

ELISA Kit for Bovine Glutathione peroxidase 3

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