ELISA Kit for Bovine Angiopoietin-related protein 1

Mfr.No.

E8972b

Alternative Names

Angiopoietin-like protein 1

Categories

Elisa

Specificity

Natural and recombinant Bovine Angiopoietin-related protein 1

Subcellular Location

Secreted

Entryname

ANGL1_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-103%	92-105%	93-107%	90-104%
EDTA plasma(n=5)	90-98%	91-106%	93-105%	88-103%
heparin plasma(n=5)	87-99%	92-105%	95-108%	90-109%

Platform

PF00147

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.1%

Protein Name

Angiopoietin-related protein 1

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	101	93-106
Plasma	99	90-106

Sequence

50:

MKAFIWTLSV ; LFFLLMGIGH ; GRGRQFKIKK ; ITQRRYPRAT ; DGKEDAKKCS ;

100:

YTFLVPEQKI ; TGPICVNTKG ; QDAGTIKDMI ; TRMDLENLKD ; VLSKQKREID ;

150:

VLQLVVDVDG ; NIVNEVKLLR ; KESRNMNSRV ; TQLYMQLLHE ; IIRKRDNSLE ;

200:

LSQLENKILN ; VTTEMLKMAT ; RYRELEVKYA ; SLTDLVNNQS ; VMITLLEEQC ;

250:

LRIFSRQDPH ; VSPPLVQVVP ; QHIPNSHQYT ; PGLLGGNEIQ ; RDPGYPRDLM ;

300:

PPPDLVTSPT ; KSPLKIPPVT ; FINEGPYKDC ; QHAKDAGHSV ; SGIYMIKPEN ;

350:

SNGPMQLWCE ; NSLDPGGWTV ; IQKRTDGSVN ; FFRNWENYKK ; GFGNIDGEYW ;

400:

LGLENIYLLS ; NQDNYKLLIE ; LEDWSDKKVY ; AEYSSFRLEP ; ESEFYRLRLG ;

450:

TYQGNAGDSM ; MWHNGKQFTT ; LDRDKDMYAG ; NCAHFHKGGW ; WYNACAHSNL ;

491:

NGVWYRGGHY ; RSKYQDGIFW ; AEYRGGSYSL ; RAVQMLIKPI ; D

Sequence Length

491

Uniprot	Q1RMR1
Gene Name	ANGPTL1
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:509971
Unigene	Bt.16731

Reference	1. "Genomic structure of Bos taurus angiopoietin-like proteins." Shui Y. Submitted (2008-03) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] 2. NIH - Mammalian Gene Collection (MGC) project Submitted (2006-04) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Hereford. tissue: Uterus.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800707	E8972b	96T	$900		Add to Cart

ELISA Kit for Bovine Angiopoietin-related protein 1

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