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Home / Assay Kits / ELISA Kits

ELISA Kit for Bovine 5'-AMP-activated protein kinase subunit gamma-1

SKU : BHE13800472

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E10187b
Categories	Elisa
Function	AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Specificity	Natural and recombinant Bovine 5'-AMP-activated protein kinase subunit gamma-1
Entryname	AAKG1_BOVIN
Platform	PF00571
Protein Name	5'-AMP-activated protein kinase subunit gamma-1
Sequence	50: MEAVPSSDSY ; PAVENEHLQE ; TPESNNSVYT ; SFMKSHRCYD ; LIPTSSKLVV ; 100: FDTSLQVKKA ; FFALVTNGVR ; AAPLWDSKKQ ; SFVGMLTITD ; FINILHRYYK ; 150: SALVQIYELE ; EHKIETWREV ; YLQDSFKPLV ; CISPNASLFD ; AVSSLIRNKI ; 200: HRLPVIDPES ; GNTLYILTHK ; RILKFLKLFI ; TEFPKPEFMS ; KSLEELQIGT ; 250: YANIAMVRTT ; TPVYVALGIF ; VQHRVSALPV ; VDEKGRVVDI ; YSKFDVINLA ; 300: AEKTYNNLDV ; SVTKALQHRS ; HYFEGVLKCY ; LHETLETIIN ; RLVEAEVHRL ; 330: VVVDENDVVK ; GIVSLSDILQ ; ALVLTGGEKP ;
Sequence Length	330
String	P58108
Sub Unit	AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

Uniprot	P58108
Gene Name	PRKAG1
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:282324
Unigene	Bt.5588

Reference	1. "Characterization of the bovine AMPK gamma-1 gene." Benkel B., Kollers S., Fries R., Sazanov A., Yoshida E., Davoren J., Hickey D. Submitted (2000-12) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 2. "Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics6:166-166(2005) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] 3. NIH - Mammalian Gene Collection (MGC) project Submitted (2006-02) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Hereford. tissue: Uterus.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13800472	E10187b	96T	$900		Add to Cart

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