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Home / Assay Kits

Easy Protein Assay Reagent

BHE16300260

This product includes 100 ml of 5 x reagent. It is for 2500 assays using 96-well plate or 500 assays using 1 ml-cuvette.

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Tel
+1 866.986.9598
Email
[email protected]
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Product Manual Datasheet
Protocols Description PROTOCOL
(1) Diluted 1 ml of the reagent with 4 ml of water.
(2) Mix 200 µl of the diluted reagent with 20 µl of the protein solution for measurement using 96-well plates. For measurements using 1 ml- cuvettes, mix 1 ml of the diluted reagent with 0.1 ml of the protein solution.
(3) Read the light absorbance at 595 nm (A595).
 
Data Analysis
Plot the A595 and the protein concentration [Protein] to generate the linear standard curve.
 
                                                          A595 = a [Protein] + b
 
Where the A595values are from experimental data, the a and b values are from the linear fitting between the A595 values and the protein concentrations.
 
UNKNOWN SAMPLES
Follow the same procedure to measure the A595 values from the unknown samples. Calculate the protein concentrations in the unknown samples using the A595values from the unknown samples and the a and b values from the standard curve.
 
                                                        [Protein] = (A595 – b) / a
SKU Supplier No. Size Your price Quantity
BHE16300260 EPA001 2500 assays $140.38
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