JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Assay Kits

E. coli NAD+-dependent DNA ligase assay kit Plus

SKU : BHE16300073

This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T, 10 ul of 500 x E. coli ligase and 220 ul of 10 x fluorescence dye for 100 assays.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

ProFoldin

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	NLA100KE, NLA100K
Description	NAD+ -dependent DNA ligases are present in bacteria, some entomopox viruses and mimi virus. Since NAD+ -dependent DNA ligases are essential for bacterial growth, they are valuable targets for identifying novel antibacterial agents. The NAD+ -dependent DNA Ligase Assay Kit is to measure the DNA ligase product in which the diphosphodiester bond is formed at the single stand break of a duplex DNA substrate. The ligase reaction is monitored by the fluorescence intensity at 535 nm. The assay is in 96-well plate format and can be used for screening inhibitors of DNA ligases from gram-positive (such as S. pneumoniae) and gram-negative (such as E. coli) bacteria. The E. coli NAD+ -dependent DNA Ligase Assay Kit Plus (Catalog number NLA100KE) includes 800 µl of 10 x buffer, 44 µl of 100x DNA substrate, 44 µl of 100 x NAD+ (10 mM), 21 ml of Reagent T, 10 µl of 500 x E. coli ligase (500 nM) and 220 µl of 10 x fluorescence dye for 100 assays.
Drug target assays	Antibacterial drug targets

Product Manual	Datasheet
Protocols Description	Assay Protocol 1.Reagent preparation: 10 x DNA: dilute the 100 x DNA with water 100 x enzyme: 100 nM NAD+ -dependent DNA ligase diluted form the 500 nM stock with 1 x Buffer 1 mM NAD+ : dilute the 10 mM stock with water 1 x fluorescence dye: dilute the 10 x fluorescence dye 10-fold with water 2. Reaction: The total volume of each reaction mixture is 40µl including: 27.6µl of H2O, 4µl of 10 x buffer (Buffer LS), 4µl of 10 x DNA, 0.4µl of 100 x enzyme, and 4µl of 1 mM NAD+ . Incubate the reaction mixture at 37°C for 60 min. 10 x Buffer LS: 200 mM HEPES, pH 7.5, 400 mM NH4Cl, 20 % glycerol, 20 mM DTT, 0.05 % Tween-20, 5 mM EDTA, 0.6 mg/ml BSA, 80 mM MgCl2. 3. Detection: Add 200µl of Reagent T into the 40µl of reaction mixture. Then add 20µl of the 1 x fluorescence dye. Mix the reaction solution and incubate it for 15 min. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

Combination SKU	Supplier No.	Specifications	Your price	Quantity
BHE16300073-1	NLA100KE	E. coli ligase included	$556.13		Add to Cart
BHE16300073-2	NLA100K	enzyme not included	$438.43		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.