Dilutethe 10% tissue homogenate to different concentrations of 2%, 1%, and 0.5%, thentake 100 uL for pre-experiment according to the operation steps. The OD valueof sample tube should be less than 1.0.The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor, 10% Animal tissue homogenate 5, GES-1 cells (2.52mg/mL) 1. Note: The diluent is normalsaline (0.9% NaCl).
Storage
Reagent 1:4°CReagent 2:4°C
Valid Period
12 Months
Reviews of Direct bilirubin (D-BIL) Colorimetric Assay Kit (Chemical Oxidation Method)