JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.
Loding Loading ....
Home / Assay Kits

Detergent assay kit

BHE16300266

This product includes 1 ml of 100 x dye A43, 100 ml of Reagent 1, 0.5 ml of 100 x Reagent 2. It is for measurement of 1000 samples using 96-well plates. A cuvette may also be used for the measurement of detergent concentrations.

Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Request More Infos
Order Now

Credit card payments now incur a 3% fee.

Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Ask Merchant
Request More Infos
Sample Request
Product Manual Datasheet
Protocols Description ASSAY PROTOCOL
The following protocol is for assays using a standard black 96-well plate (Costar 3915 or Greiner 655076). Adjust the assay reagent volumes proportionally for measurements using cuvettes.
 
1. Prepare standard detergent solutions with a series of concentrations from 0.001% to 0.1% in water or a buffer. Prepare 1 x dye A43 by dilution of the 100 x dye A43 with Reagent 1 (100-fold dilution). Each assay needs 100 µl of 1 x dye A43. Prepare 1 x Reagent 2 by dilution of the 100 x Reagent 2 with water (100 fold dilution). Each assay needs 50 µl of 1 x Reagent 2.
 
2. Mix 50 µl of the detergent solution with 100 µl of 1 x dye A43. Then add 50 µl of 1 x Reagent 2 and mix the solution for 2 min.
 
3. Read the fluorescence at 535 nm (excitation at 485 nm). Plot the correlation between the fluorescence values and the detergent concentrations and compare the data from the unknown samples.
SKU Supplier No. Size Your price Quantity
BHE16300266 DAK1000 1000 assays $358.75
Add to Cart