Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 100 cells can be accurately quantified.Time efficient. High-throughput assay in 96-well and 384-well plates allows simultaneous processing ten of thousands of samples per day.Homogeneous and convenient. A single reagent and "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS. Z factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.
Intended Use
FL530/590nm
Detection Principle
100 cells
Shelf Life
12 months
Specifity
Homogeneous assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents.
Claus, J.,et al. (2020). Swelling characteristics and biocompatibility of ionic liquid based hydrogels for biomedical applications. PLOS ONE, 15(4), e0231421 Assay: Cell Viability in mouse fibroblast cells.
Harmon, N. M.,et al. (2020). Potent double prodrug forms of synthetic phosphoantigens. Bioorganic & Medicinal Chemistry, 28(19), 115666. Assay: Cell Viability in human PBMC cells.
Miyamoto K et al (2019). Synergistic Effects of Olaparib and DNA-damaging Agents in Oesophageal Squamous Cell Carcinoma Cell Lines. Anticancer Res. 39(4):1813-1820. Assay: Cell viability in human cell lines.
Choi EH et al (2018). Activated T cells induce proliferation of oligodendrocyte progenitor cells via release of vascular endothelial cell growth factor-A. Glia.66(11):2503-2513. Assay: Cell viability in human hematopoietic stem cells.
Minegaki, T (2018). Augmentation of the cytotoxic effects of nitrogen-containing bisphosphonates in hypoxia. J Pharm Pharmacol. 70(8):1040-1047. Assay: Cell viability in human cancer cells.
Minegaki, T et al (2018). Histone deacetylase inhibitors sensitize 5-fluorouracil-resistant MDA-MB-468 breast cancer cells to 5-fluorouracil. Oncol Lett. 16(5):6202-6208. Assay: Cell viability in human breast cancer cell line.
Uchiyama H et al (2018). Evaluation of Trace Elements in Augmentation of Statin-Induced Cytotoxicity in Uremic Serum-Exposed human Rhabdomyosarcoma Cells. Toxins (Basel). 10(2). pii: E53. Assay: Cell viability in human cells.
Wulf K (2018). Novel approach for a PTX/VEGF dual drug delivery system in cardiovascular applications-an innovative bulk and surface drug immobilization. Drug Deliv Transl Res. 8(3):719-728. Assay: Cell viability in human epithelial cells.
Megumi C et al (2017). Preventive Activity of ar-Turmerone and Bisacurone Isolated from Turmeric Extract Against Ethanol-induced Hepatocyte Injury. Food Science and Technology Research, 23 (2), 275-281. Assay: Cell viability in mice hepatocytes.
Tsujimoto M et al (2017). Inhibitory Effects of Juices Prepared from Individual Vegetables on CYP3A4 Activity in Recombinant CYP3A4 and LS180 Cells. Biol Pharm Bull. 40(9):1561-1565. Assay: Cell viability in human cell lines.
Yan H, Ajuwon KM (2017). Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway. PLoS One. 12(6):e0179586. Assay: Cell viability in pig epithelial cells.
Chen, X. et al (2016). Cytotoxicity of various chemicals and mycotoxins in fresh primary duck embryonic fibroblasts: a comparison to HepG2 cells. J Appl Toxicol. 36(11):1437-45. Assay: Cell viability in duck embryonic fibroblasts.
Ibrihim, MIM et al (2014). Aqueous extract of Corchorus olitorius decreases cytotoxicity of aflatoxin B atoxin B1and fumonisin B and fumonisin B1 in H4IIE- in H4IIE-luccells.Hepatoma Research. 1(2). Assay: Cell viability in rat hepatoma cells.
Fujimoto, M et al (2011). Examination of Cell Viability using the primary culture system of skin fibroblasts. Cell Viabilityof skin fibroblast 2: 23-26. Assay: Cell viability in human fibroblasts.
Hemendinger RA,et al (2011). Methyl Vitamin B12 but not methylfolate rescues a motor neuron-like cell line from homocysteine-mediated cell death. Toxicol Appl Pharmacol. 251(3):217-25. Assay: Cell viability in human cell lines.
Hahn A et al (2010). Therapeutic window for bioactive nanocomposites fabricated by laser ablation in polymer-doped organic liquids. Adv. Eng. Mater. 12(5). Assay: Cell viability in mouse cell lines.
Mitsui R, et al (2010). Maintenance of paracellular barrier function by insulin-like growth factor-I in submandibular gland cells. Arch Oral Biol. 55(12):963-9. Assay: Cell viability in rat cells.
Ramirez, CN, et al (2010). Cell viability assessment: toward content-rich platforms. Expert Opinion on Drug Discovery 5(3): 223-233. Assay: Cell viability in human cell line.
Heminger K, et al (2009). Alterations in gene expression and sensitivity to genotoxic stress following HdmX or Hdm2 knockdown in human tumor cells harboring wild-type p53. Aging 1(1):89-108. Assay: Cell viability in human cell lines.
Nakajima Y,et al (2009). Neuroprotective effects of Brazilian green propolis and its main constituents against oxygen-glucose deprivation stress, with a gene-expression analysis. Phytother Res. 23(10):1431-8. Assay: Cell viability in rat cell line.
Zayas-Santiago A, Kang Derwent JJ (2009). Preservation of intact adult rat photoreceptors in vitro: study of dissociation techniques and the effect of light. Mol Vis.15:1-9. Assay: Cell viability in rat photoreceptor cells.
Desplats PA, et al (2008). Functional roles for the striatal-enriched transcription factor, Bcl11b, in the control of striatal gene expression and transcriptional dysregulation in Huntington's disease. Neurobiol Dis. 31(3):298-308. Assay: Cell viability in human cells.
Hemendinger RA, et al (2008). Huperzine A provides neuroprotection against several cell death inducers using in vitro model systems of motor neuron cell death. Neurotox Res.13(1):49-61. Assay: Cell viability in rat cell lines.
Vijaykumar TS, et al (2008). Chloroquine mediated molecular tuning of astrocytes for enhanced permissiveness to HIV infection. Virol. 381(1):1-5. Assay: Cell viability in human cells.
Mochida S et al (2007). Geranylgeranylacetone Ameliorates Inflammatory Response to Lipopolysaccharide (LPS) in Murine Macrophages: Inhibition of LPS Binding to The Cell Surface. J. Clin. Biochem. Nutr. 41(2):115-23. Assay: Cell viability in mouse cell lines.
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