BeaverBeads™ Protein A for Immunprecipitation

Mfr.No.

22203-20, 22203-100

Description

BeaverBeadsTM Protein A / G Matrix beads immunoprecipitation product enables Protein A / G to cover superparamagnetic microspheres with high density by using Beaver biological nano- technology. Comparing to similar products currently on the international immunomagnetic beads market, it has more antibody binding points, less usage of beads and low binding rate with other protein, so it makes immunoprecipitation experiments more efficient. Each milliliter immunoprecipitation beads can bind with human IgG for more than 300μg, and a single precipitation reaction only need 25μL beads. The super-large specific surface area provided by the micrometer beads can significantly reduce the equilibrium time required for adsorption of antibody and antigen. The antibody adsorption process can be completed within 15 min and the antigen precipitation process is completed within 30 min. Short operating time can avoid target protein hydrolysis, ensuring the activity of the target protein and integrity of protein complex. This product can be widely used in cell lysate, cell secretion supernatant, blood plasma, ascites, tissue culture supernatant and other samples of antigen immunoprecipitation reaction. The operator can refer to the operating instructions, the data in Table 1 can be used to understand the binding capacity between different species and subtypes of antibodies and Protein A beads, Protein A/G.

Notes

1. Be sure to read this operating instruction carefully before performing an immunoprecipitation operation.
2. This product must be used with the magnetic separator.
3. Beads should be fully oscillated before use.
4. The beads should be stored in the preservation to prevent drying.
5. Do not freeze or centrifuge the beads to avoid irreversible aggregation.
6. To ensure the best results, select a strong specificity antibody for immunoprecipitation.
7. The operator can detect the binding situations of antibodies, antigens and beads using the supernatant collected in the antigen binding reaction step according to the actual requirements.
8. For IP experiments, the affinity of different types of antibodies to antigen binding is different, and the binding of the antibody to the antigen will also be affected by the binding buffer and the washing buffer. If the operator does not obtain good experimental results using the buffer system provided by this kit, he or she can screen and configure the buffer for the experiment.
9. 10×PBS (③) should dilute in the sterile environment, once find the solution having a contamination, please stop use this solution.
10. The coated protein A / G on the surface of this bead has few protein shedding situations under extreme conditions (such as low pH, heat treatment), but it is not advisable for the operator to use a protein with a molecular weight of about 130 kD Immunoprecipitation experiments.
11. This product is for research use only.

Product Information

Products name	BeaverBeads™ Protein A (or A/G) Immunoprecipitation Kit
BeaverBeadsTM Protein A/G for IP ①	1 mL
IP Binding Buffer ②	30 mL
phosphate Buffer PBS (10×, dilute to 1×before use) ③	20 mL
IP Washing Buffer ④	20 mL
IP Elution Buffer ⑤	0.5 mL
IP Neutralization Buffer ⑥	0.2 mL
Magnetic Separator Stand 2/15 ⑦	optional

Note: the capacity binding of Human IgG Antibody Capacity are: Protein A： 0.4~0.5 mg/mL; Protein A/G： 0.5~0.6 mg/mL

Shelf Life

1 year

Storage	2~8℃ for long-term preservation

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Combination SKU	Supplier No.	Specification	Your price	Quantity
BHE13200002-1	22203-20	10mg/mL, 20rnxs	$113		Add to Cart
BHE13200002-2	22203-100	10mg/mL, 100rnxs	$330		Add to Cart

Datasheet

BeaverBeads™ Protein A for Immunprecipitation

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