Alpha B Crystallin ELISA Kit

Mfr.No.	SKT-123
Alternative Names	AACRYA ELISA Kit, CRYA2 ELISA Kit, CRYAB ELISA Kit, CTPP2 ELISA Kit, HSPB5 ELISA Kit, NY Ren 27 antigen ELISA Kit, alpha crystallin B ELISA Kit, AACRYA ELISA Kit ; CRYA2 ELISA Kit ; CRYAB ELISA Kit ; CTPP2 ELISA Kit ; HSPB5 ELISA Kit ; NY Ren 27 antigen ELISA Kit ; alpha crystallin B ELISA Kit
Categories	ELISA Kits
Description	Colorimetric detection of alpha B Crystallin
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	500
Reactivity	Human
Scientific Background	The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. alpha and beta families are further divided into acidic and basic groups (alpha-A and alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
Size	1 Plate ; 1 vial/10 ml ; 2 vials ; 1 vial/ 50 ml ; 1 vial/100 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/150 µl ; 1 vial/ 13 ml ; 1 vial/ 13 ml ; 1 vial/ 13 ml
Adr Code	Non-hazardous
Assay Overview	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Assay Range	0.352 - 22.5 ng/ml
Assay Type	Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Assay Type Abbreviation	Sandwich
Component Number	SKC-123A ; SKC-123B ; SKC-123C ; SKC-123D ; SKC-123E ; SKC-123F ; SKC-123G ; SKC-123H ; SKC-123I ; SKC-123J ; SKC-123K
Country Of Origin	Canada
Detection Method	Colorimetric Assay
Detection Range	0.352 - 22.5 ng/ml
Incubation Time	30 minutes
Item	Anti-Alpha B Crystallin Immunoassay Plate ; 5X Alpha B Crystallin Extraction Reagent ; Recombinant Alpha B Crystallin Standard ; Standard and Sample Diluent ; 10X Wash Buffer Concentrate ; Anti-Alpha B Crystallin Biotinylated Antibody Concentrate ; Anti-Alpha B Crystallin Biotinylated Antibody Diluent ; Streptavidin: HRP Concentrate ; Streptavidin: HRP Diluent ; TMB Substrate ; Stop Solution
Method Type	Cell Lysates, Tissue, Serum
Number Of Samples	40 samples in duplicate
Platform	Microplate
Sensitivity	0.009 ng/ml
Species Reactivity Abbreviations	Hu
Species Reactivity Medium	Human
Trademark	StressXpress®

Research Area	Cancer Heat Shock
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature

Blue Ice

Description

Trademark: StressXpress®
Platform: Microplate
Utility: ELISA kit used to quantitate alpha B crystallin concentration in samples.
kit-overview:

Component No.	Item	Quantity / Size
SKC-123A	Anti-Alpha B Crystallin Immunoassay Plate	1 Plate
SKC-123B	5X Alpha B Crystallin Extraction Reagent	1 vial/10 ml
SKC-123C	Recombinant Alpha B Crystallin Standard	2 vials
SKC-123D	Standard and Sample Diluent	1 vial/ 50 ml
SKC-123E	10X Wash Buffer Concentrate	1 vial/100 ml
SKC-123F	Anti-Alpha B Crystallin Biotinylated Antibody Concentrate	1 vial/150 µl
SKC-123G	Anti-Alpha B Crystallin Biotinylated Antibody Diluent	1 vial/ 13 ml
SKC-123H	Streptavidin: HRP Concentrate	1 vial/150 µl
SKC-123I	Streptavidin: HRP Diluent	1 vial/ 13 ml
SKC-123J	TMB Substrate	1 vial/ 13 ml
SKC-123K	Stop Solution	1 vial/ 13 ml

Incubation Time: 30 minutes
Shipping Temperature: Blue Ice
Gross Weight (kg): 2
Net Weight (g): 500
Stock Product: Y
Country of Origin: Canada

Storage

4ºC

Storage Temperature

4ºC

Stock Product

Utility

ELISA kit used to quantitate alpha B crystallin concentration in samples.

Reference	1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577. 9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.

Protocols Description	1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Other Resources 1	Kit Booklet
Other Resources 2	MSDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE11900019-1	SKT-123-480	5 X 96 well	$3071		Add to Cart
BHE11900019-2	SKT-123-96	96 well	$723		Add to Cart

Alpha B Crystallin ELISA Kit

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