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Elabscience Biotechnology Co.,Ltd
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Elabscience Biotechnology Co.,Ltd
view supplier detailsCredit card payments now incur a 3% fee.
| Mfr.No. | E-BC-K235-M |
| Alternative Names | Glutamic-pyruvic transaminase |
| Description | This kit can be used to measure ALT/GPT activity in animal serum (plasma), tissue, culture cells and cell culture fluid. |
| Description | Biochemical |
| Assay Type | Biochemical kit |
| Detection Instrument | Microplate reader |
| Detection Method | Colorimetric method |
| Detection Principle | Glutamic-pyruvic transaminase (Alanine aminotransferase /ALT /GPT) can react with the substrate consisted of alanine and α-ketoglutaric acid in pH7.4 solution at 37℃, and then produce alanine and glutamic acid. Add 2,4-dinitrophenylhydrazine (DNPH) hydrochloric acid solution after 30 min to stop the reaction. Then the addition reaction occurs between DNPH and the carbonyl in ketonic acid and produce pyruvic acid hydrazine. The phenylhydrazone can show brown color in alkaline condition. Measure the OD values at 505 nm and calculate the enzyme activity. |
| Detection Range | 0.75-72.3 IU/L |
| Experiment Instruments | Microplate reader |
| Inter-cv (%) | 9 |
| Intra-cv (%) | 5.3 |
| Manual | 
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| Sensitivity | 0.75 IU/L |
| Manual | 
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| SKU | Supplier No. | Size | Your price | Quantity | |
|---|---|---|---|---|---|
| BHE10307679 | E-BC-K235-M | 96T | $180 |
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| BHE10307679 | E-BC-K235-M | 48T | $120 |
|
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