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Home / Assay Kits / Apoptosis & Proliferation

Caspase-1 Assay Kit (Colorimetric)

SKU : BHE13700021

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTA3020
Background	Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase-1 (ICE, interleukin-1beta-converting enzyme) is the mammalian caspase responsible for the proteolytic conversion of the proforms of two inflammatory cytokines, interleukin-1beta and IL-18 into active cytokines, initiating a proinflammatory response. It also cleaves Gasdermin D into its active form, which leads to pyroptosis.
Description	Caspase-1 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-1.
Featured	Simple and optimized procedure. Fast and convenient. Detect early/middle stages of apoptosis.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Tag	Caspase
Description	Caspase 1 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-YVAD-pNA (acetyl-Tyr-Val-Ala-Asp p-nitroanilide) by caspase 1, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.
Kit Components	Cell Lysis Buffer Reaction Buffer (2x) Ac-YVAD-pNA (4mM) pNA(10mM) DTT (100x)
Notes	Caspase 1 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-YVAD-pNA (acetyl-Tyr-Val-Ala-Asp p-nitroanilide) by caspase 1, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.

Shipping	Gel pack with blue ice.
Storage Instructions	Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.

Applications	Cellular states

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700021	KTA3020	20 T	$69		Add to Cart
BHE13700021	KTA3020	50 T	$129		Add to Cart
BHE13700021	KTA3020	100 T	$249		Add to Cart

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