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Home / Assay Kits / Apoptosis & Proliferation

Annexin V-AbFluor™ 647 Apoptosis Detection kit (Red Fluorescence)

SKU : BHE13700010

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTA0004
Alternative Names	Annexin 5; Annexin V; AbFluor 647
Background	Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the exteal cellular environment. The human anticoagulant, Annexin V, is a 3536 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red ?uorescence.
Description	Abbkine Annexin V-AbFluor 647 Apoptosis Detection Kit allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.
Featured	Abbkine AbFluor 647 dye Provides the same or better fluorescence and photostability as Alexa Fluor 647, Cy5, DyLight 649 Annexin V- AbFluor 647: Abs/Em = 650/665 nm; PI: Abs/Em = 535/617 nm (with DNA) Suitable for flow cytometry or fluorescence microscopy
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Tag	Annexin V-AbFluor 647
Description	Abbkine Annexin V-AbFluor™ 647 Apoptosis Detection Kit contains Annexin V labeled with Abbkine proprietary far-red fluorescent dye AbFluor™ 647, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor™ 647 and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.
Kit Components	Annexin V Binding Buffer (5x) Annexin V- AbFluor 647 Propidium Iodide (PI)
Notes	Abbkine Annexin V-AbFluor 647 Apoptosis Detection Kit contains Annexin V labeled with Abbkine proprietary far-red fluorescent dye AbFluor 647, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor 647 and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.

Shipping	Blue ice
Storage Instructions	Stable for at least 6 months at 4°C from date of shipment. Protect from light and do not freeze.

Applications	Cellular states

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700010	KTA0004	50 T	$149		Add to Cart
BHE13700010	KTA0004	100 T	$259		Add to Cart

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