Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation.
Expression System
Yeast
Protein Nature
Recombinant
Shape
Liquid,dissolved in 20mM Tris-HCl, 500mMNaCl, pH 8.0
Tag
his
Resarch Areas
Autoimmune Disease
Immunogen Species
Homo Sapiens (Human)
Uniprot
Q53957
Purity
>90% by SDS-PAGE
Storage
Aliquot and store at -20 ° C or -80 ° C. Avoid repeated freeze / thaw cycles