ELISA, WB, IHC, Immunoinhibition Method and other possible application.
Applications
WB
Active
Measurement of the cAST activities in biochemistry platform. The change of absorbance can be determined turbidimetrically at 340 nm over a fixed-time interval.
Aspartate aminotransferase exists in two isoenzymic forms-mitochondrial (m-AST) and cytosolic (c-AST),which is present in most organs,and the highest concentrations are found in the liver, heart, skeletal muscle, kidneys, brain and red blood cells. Because of its wide tissue distribution, elevated AST levels have low specificity for any single disease. AST has been used clinically to diagnose hepatitis, myocardial infarction and skeletal muscle disease. Serumm-AST, serum AST and their ratio (m-AST/AST) are measured clinically as biomarkers for liver diseases and the damage of mitochondria associated with hepatocellular damage, such as acute and chronic viral hepatitis, fulminant hepatitis, chronic active hepatitis and cirrhosis.
Expression System
E.coli
Protein Nature
Recombinant
Shape
Supplied in liquid form in 20 mM Tris-HCl, 500 mM NaCl, pH 8.0, 50% glycerol
Tag
his
Resarch Areas
Liver Function & Injury
Immunogen Species
Homo Sapiens (Human)
Uniprot
P46248
Purity
>90% by SDS-PAGE
Storage
Aliquot and store at -4°C. Avoid repeated freeze / thaw cycles
Reviews of Human Aspartate aminotransferase, cytoplasmic