Mouse Anti-Human VImentin Monoclonal IgG1

Mfr.No.	SMC-261, SMC-261D, SMC-261D-A390, SMC-261D-A488, SMC-261D-A594, SMC-261D-BI, SMC-261D-FITC
Immunogen Species	Human
Alternative Names	CTRCT30 Antibody, Epididymis luminal protein 113 Antibody, HEL113 Antibody, VIM Antibody, VIME_HUMAN Antibody, Vimentin Antibody
Antigen	Vimentin
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	A 1:2000 dilution of SMC-261 was sufficient for detection of Vimentin in 10 ug of Human, Mouse, Rat, HeLa, Brain cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC
Description	Mouse Anti-Human Vimentin Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Full-length protein., Homo Sapiens (Human)
Immunogen Region	Full-length Human Vimentin protein
Isotype	IgG1
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	P08670
Accession Number	NP_003371.2
Gene Id	7431
Target	Vimentin
Clone No	1A7
Research Area	Neurogenesis, Cell Signaling, Intermediate Filaments, Cell Markers, Neuron Markers, Neural Stem Cell Markers, Stem Cells, Lineage Markers, Endoderm, Cancer, Tumor Biomarkers, Neuroregeneration, Neuroscience, Vimentin, Cytoskeleton

Buffer	PBS, pH 7.4, 50% glyercol, 0.05% sodium azide
Dilutions	WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Vimentin is a type III intermediate filament (IF) protein. All IF proteins are highly expressed and vimentin is the major cytoskeletal component of mesechymal, and therefore Vimentin is used as a marker of mesenchymally-derived cells or cells undergoing EMT during both normal development and metastic progression. Functionally it also serves a significant role in supporting and anchording the organelles in the cytosol. Clinical significance includes being used as a sarcoma tumor marker, and it has also been used in the diagnosis of rheumatoid arthritis.
Shipping Temperature	Blue Ice or 4°C
Specificity	Antibody can detects endogenous Vimentin protein.
Storage	-20ºC
Storage Buffer	PBS, pH 7.4, 50% glyercol, 0.05% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Katsumoto T., Mitsushima A., Kurimura T. (1990). Biol Cell. 68(2): 139146. 2. Leader M, Collins M, Patel J, Henry K (1987). Histopathology 11(1): 6372. 3. Immunohistochemistry from the Washington Animal Disease Diagnostic laboratory (WADDL)of the College of Veterinary Medicine, Washington State University. Retrieved 2009-03-14.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11902273-1	SMC-261D	Unconjugated	100 ul	$286	Add to Cart
BHA11902273-2	SMC-261D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11902273-3	SMC-261D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11902273-4	SMC-261D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11902273-5	SMC-261D-BI	Biotin	100 ul	$334	Add to Cart
BHA11902273-6	SMC-261D-FITC	FITC	100 ul	$330	Add to Cart

Vimentin Antibody, Clone 1A7 - SMC-261