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Home / Primary Antibodies

Ubiquitin Antibody (V3894SAF)

SKU : BHA17125713

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NSJ Bioreagents

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Mfr.No.	V3894SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Ubiquitin is a small (8.5 kDa) regulatory protein found in most tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or less frequently ubiquitylation. Ubiquitination affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitination involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The result of this sequential cascade is to bind ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond. (Wiki)
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	A portion of amino acids 1-119 from the human protein was used as the immunogen for the Ubiquitin antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	The stated application concentrations are suggested starting points. Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Uniprot	P0CG47, P0CG48, P62979, P62987, P62988

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Ubiquitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IHC-P
Description	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125713	V3894SAF-100UG	100 ug	$499		Add to Cart

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