Mouse Anti-Human Ubiquitin Monoclonal IgG1

Mfr.No.	SMC-214, SMC-214D, SMC-214D-A390, SMC-214D-A488, SMC-214D-A594, SMC-214D-ALP, SMC-214D-APC, SMC-214D-BI, SMC-214D-FITC, SMC-214D-HRP, SMC-214D-PCP, SMC-214D-RPE
Immunogen Species	Human
Alternative Names	Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm, Nucleus
Certificate Of Analysis	A 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 ug of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Human Ubiquitin Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Ubiquitin conjugated lysozyme, Homo Sapiens (Human)
Immunogen Region	Human Ubiquitin conjugated lysozyme
Isotype	IgG1
Launch Date	2014/06/12
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Species Independent

Swissprot	P0CG47
Accession Number	BAC56955.1
Gene Id	7314
Target	Ubiquitin
Clone No	FK2
Research Area	Cell Signaling, Post-translational Modifications, Ubiquitination, Neurodegeneration, Alzheimer's Disease, Neuroscience

Buffer	10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide
Concentration	10 mg/ml
Dilutions	WB (1:10000); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Scientific Background	Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
Storage	-20ºC
Storage Buffer	10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IHC, IP, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution	WB (1:10000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12. 2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11901793-1	SMC-214D	Unconjugated	100 ug	$174	Add to Cart
BHA11901793-2	SMC-214D-A390	ATTO 390	100 ug	$228	Add to Cart
BHA11901793-3	SMC-214D-A488	ATTO 488	100 ug	$227	Add to Cart
BHA11901793-4	SMC-214D-A594	ATTO 594	100 ug	$227	Add to Cart
BHA11901793-5	SMC-214D-ALP	Alkaline Phosphatase	100 ug	$220	Add to Cart
BHA11901793-6	SMC-214D-APC	APC	100 ug	$226	Add to Cart
BHA11901793-7	SMC-214D-BI	Biotin	100 ug	$222	Add to Cart
BHA11901793-8	SMC-214D-FITC	FITC	100 ug	$218	Add to Cart
BHA11901793-9	SMC-214D-HRP	HRP	100 ug	$213	Add to Cart
BHA11901793-10	SMC-214D-PCP	PerCP	100 ug	$226	Add to Cart
BHA11901793-11	SMC-214D-RPE	RPE	100 ug	$223	Add to Cart

Ubiquitin Antibody, Clone FK2 - SMC-214