The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
A portion of amino acids 300-400 from Type II Cytokeratins was used as the immunogen for the recombinant Type II Cytokeratin antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
Optimal dilution of the recombinant Type II Cytokeratin antibody should be determined by the researcher.
Uniprot
Q01546
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the recombinant Type II Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT