TNF (tumor necrosis factor) encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Clonality
Monoclonal
Description
Mouse Anti-TNF alpha Monoclonal Antibody
Formulation
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen, Liquid solution
Host
Mouse
Immunogen
Synthetic Peptide
Immunogen Region
Synthetic Peptide
Isotype
Mouse IgG1
Molecular Weight
17, 25 KD
Others
The antibody detects TNF alpha.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Reactivity
Human, Mouse, Rat
Tag
TNF alpha
Note
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000), IHC-P (1:50-1:200).
Accession Number
P01375
Gene Id
7124, 7124
Concentration
1 mg/ml, 1 mg/ml
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen, The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen
Storage Buffer
PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage Instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.