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Home / Primary Antibodies

TNF alpha Antibody (V3719SAF)

SKU : BHA17125515

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NSJ Bioreagents

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Mfr.No.	V3719SAF-100UG
Clonality	Recombinant Rabbit Monoclonal
Description	This mAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Rabbit
Immunogen Region	Recombinant human protein was used as the immunogen for the recombinant TNFa antibody.
Isotype	Rabbit IgG, kappa
Species Reactivity	Human
Note	Optimal dilution of the recombinant TNFa antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P01375

Format	Purified
Purity	Protein A affinity chromatography
Storage	Store the recombinant TNFa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 4-8ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125515	V3719SAF-100UG	100 ug	$499		Add to Cart

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