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Home / Primary Antibodies

TNF alpha Antibody (V2905SAF)

SKU : BHA17124491

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NSJ Bioreagents

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Mfr.No.	V2905SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This mAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant human protein was used as the immunogen for the TNFa antibody.
Isotype	Mouse IgM, kappa
Species Reactivity	Human
Note	Optimal dilution of the TNF-alpha antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 mi

Uniprot	P01375

Format	Purified
Purity	PEG precipitation
Storage	Store the TNFa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	Neutralization, FACS, IF
Description	Neutralization (order BSA/sodium azide-free format),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124491	V2905SAF-100UG	100 ug	$499		Add to Cart

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