Rabbit Anti-Human SOD (Cu/Zn) Polyclonal

Mfr.No.	SPC-116, SPC-116D, SPC-116C, SPC-116D-A390, SPC-116D-A488, SPC-116D-A594, SPC-116D-ALP, SPC-116D-APC, SPC-116D-BI, SPC-116D-FITC, SPC-116D-HRP, SPC-116D-PCP, SPC-116D-RPE, SPC-116S
Immunogen Species	Human
Alternative Names	Superoxide dismutase1 Antibody, ALS1 Antibody, IPOA Antibody, SOD1 Antibody, SOD2 Antibody, SODC Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	0.2 ug/ml of SPC-116 was sufficient for detection of Cu/Zn SOD in 20 ug of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human SOD (Cu/Zn) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Human Cu/Zn SOD, Homo Sapiens (Human)
Immunogen Region	Synthetic Human Cu/Zn SOD Peptide
Launch Date	2007/06/01
Net Weight (g)	0.1
Reactivity	Coral, African clawed frog, Mollusk, Mussel, Dog, Pig, Rat, Fish, Sheep, Mouse, Human, Monkey, Bovine, Rabbit, Hamster, Invertebrate
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Rat, Mouse, Bovine, Monkey, Invertebrate, Coral, Dog, Hamster, Pig, Rabbit, Sheep, Xenopus, Mollusk, Fish, Human

Swissprot	P00441
Accession Number	NP_000445.1
Gene Id	6647
Target	SOD1
Research Area	Cancer, Oxidative Stress, Cell Signaling, Chaperone Proteins, Neurodegeneration, ALS Disease, Neuroscience, Protein Trafficking

Buffer	PBS pH7.0, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Scientific Background	Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2? to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~23kDa (human) and ~19kDa (other species).
Storage	-20ºC
Storage Buffer	PBS pH7.0, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IHC, IP, WB
Description	WB ; IHC ; IP ; ELISA
Dilution	WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O'Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11906483-1	SPC-116D	Unconjugated	100 ug	$345	Add to Cart
BHA11906483-2	SPC-116C	Unconjugated	25 ug	$171	Add to Cart
BHA11906483-3	SPC-116D-A390	ATTO 390	100 ug	$399	Add to Cart
BHA11906483-4	SPC-116D-A488	ATTO 488	100 ug	$398	Add to Cart
BHA11906483-5	SPC-116D-A594	ATTO 594	100 ug	$398	Add to Cart
BHA11906483-6	SPC-116D-ALP	Alkaline Phosphatase	100 ug	$392	Add to Cart
BHA11906483-7	SPC-116D-APC	APC	100 ug	$397	Add to Cart
BHA11906483-8	SPC-116D-BI	Biotin	100 ug	$394	Add to Cart
BHA11906483-9	SPC-116D-FITC	FITC	100 ug	$389	Add to Cart
BHA11906483-10	SPC-116D-HRP	HRP	100 ug	$385	Add to Cart
BHA11906483-11	SPC-116D-PCP	PerCP	100 ug	$397	Add to Cart
BHA11906483-12	SPC-116D-RPE	RPE	100 ug	$395	Add to Cart
BHA11906483-13	SPC-116S	Unconjugated	12 ug	$44	Add to Cart

SOD (Cu/Zn) Antibody - SPC-116