Rabbit Anti-Human Smad4 Polyclonal

Mfr.No.	SPC-1286, SPC-1286D, SPC-1286D-A390, SPC-1286D-A488, SPC-1286D-A594, SPC-1286D-BI, SPC-1286D-FITC
Immunogen Species	Human
Alternative Names	(Small) Mothers Against Decapentaplegic Antibody, Deleted in Pancreatic Carcinoma 4 Antibody, DPC 4 Antibody, hSMAD4 Antibody, JIP Antibody, MADH 4 Antibody, Mothers against decapentaplegic homolog 4 Antibody, SMAD 4 Antibody, SMAD mothers against DPP homolog 4 Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm, Nucleus
Certificate Of Analysis	A 1:1000 dilution of SPC-1286 was sufficient for detection of Smad4 in 10 ug of HeLa, Cos7, HepG2, HUVEC cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human Smad4 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthesized peptide derived from internal of Human Smad4., Homo Sapiens (Human)
Immunogen Region	Synthesized peptide derived from internal of human Smad4.
Isotype	IgG
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	Q13485
Accession Number	NP_005350.1
Gene Id	4089
Target	Smad4
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, Cytoplasmic, Cardiovascular System, Heart, Cardiogenesis, Metabolism, Metabolism processes, Hypoxia, TGF Beta

Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Dilutions	WB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-beta signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Smad4 mediates all activities through interaction with various other Smad family members.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~60kDa, endogenous levels of total Smad4 protein.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, WB
Description	WB ; ELISA
Dilution	WB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Heldin C.H. et al. (1997) Nature. 390: 465-71. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-94. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-91. 4. Whitman M. (1998) Genes Dev. 12: 2445-62. 5. Wu G. et al. (2000) Science. 287: 92-7.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11917352-1	SPC-1286D	Unconjugated	100 ul	$286	Add to Cart
BHA11917352-2	SPC-1286D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11917352-3	SPC-1286D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11917352-4	SPC-1286D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11917352-5	SPC-1286D-BI	Biotin	100 ul	$334	Add to Cart
BHA11917352-6	SPC-1286D-FITC	FITC	100 ul	$330	Add to Cart

Smad4 Antibody - SPC-1286