Rabbit Anti-Human Smad3 (pSer425) Polyclonal

Mfr.No.	SPC-1427, SPC-1427D, SPC-1427D-A390, SPC-1427D-A488, SPC-1427D-A594, SPC-1427D-BI, SPC-1427D-FITC
Immunogen Species	Human
Alternative Names	Smad3 Antibody, Mothers against decapentaplegic homolog 3 Antibody, Mad3 Antibody, SMAD3_HUMAN Antibody, DKFZp686J10186 Antibody, SMAD Antibody, HSPC193 Antibody, SMAD family member 3 Antibody, hMAD-3 Antibody, MGC60396 Antibody, MAD homolog 3 Antibody, HST17436 Antibody, JV15-2 Antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 Antibody, JV152 Antibody, Mad homolog JV15 2 Antibody, SMAD 3 Antibody, JV15 2 Antibody, DKFZP586N0721 Antibody, MADH3 Antibody, MAD, Mothers against DPP homolog 3 Antibody, hSMAD3 Antibody, MADH 3 Antibody, LDS3 Antibody, Mad protein homolog Antibody, SMA and MAD related protein 3 Antibody, LDS1C Antibody, hMAD 3 Antibody
Categories	Antibodies
Cellular Localization	Nucleus, Cytoplasm, Cytosol, Nuclear Chromatin, Nuclear Inner Membrane, Nucleoplasm, Plasma Membrane, Receptor Complex
Clonality	Polyclonal
Description	Rabbit Anti-Human Smad3 (pSer425) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic phospho-peptide from human Smad3 around the phosphorylation site of serine 425 (pSer425)., Homo Sapiens (Human)
Immunogen Region	Synthetic phospho-peptide from human Smad3 around the phosphorylation site of serine 425 (pSer425).
Isotype	IgG
Launch Date	2018/03/07
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	P84022
Accession Number	NP_001138574.1
Gene Id	4088
Target	Smad3 (pSer425)
Research Area	Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, Cytoplasmic, Cancer, Cancer Metabolism, Response to Hypoxia, Metabolism, Metabolism processes, Hypoxia, TGF Beta

Buffer	PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide
Dilutions	WB (1:250-1:1000); IHC (1:25-1:100); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Smad3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects endogenous levels of Smad3 only when phosphorylated at serine 425 (pSer425). Detects a band at ~58 kDa.
Storage	-20ºC
Storage Buffer	PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB
Description	WB ; IHC
Dilution	WB (1:250-1:1000); IHC (1:25-1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11919872-1	SPC-1427D	Unconjugated	100 ul	$331	Add to Cart
BHA11919872-2	SPC-1427D-A390	ATTO 390	100 ul	$385	Add to Cart
BHA11919872-3	SPC-1427D-A488	ATTO 488	100 ul	$384	Add to Cart
BHA11919872-4	SPC-1427D-A594	ATTO 594	100 ul	$384	Add to Cart
BHA11919872-5	SPC-1427D-BI	Biotin	100 ul	$380	Add to Cart
BHA11919872-6	SPC-1427D-FITC	FITC	100 ul	$375	Add to Cart

Smad3 Antibody (pSer425) - SPC-1427

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