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Home / Primary Antibodies / Monoclonal Antibodies

Rhodopsin Antibody, Clone 1D4 - SMC-177

BHA11901195
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Email
[email protected]
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Swissprot P02699
Accession Number NP_001014890.1
Gene Id 509933
Target Rhodopsin
Clone No 1D4
Research Area Neurotransmitter Receptors, Cell Signaling, Rhodopsins, Neuroscience
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).
Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61.

2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867.

3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.

4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742.

5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11901195-1 SMC-177D Unconjugated 100 ug $330
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BHA11901195-2 SMC-177C Unconjugated 25 ug $179
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BHA11901195-3 SMC-177D-A390 ATTO 390 100 ug $384
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BHA11901195-4 SMC-177D-A488 ATTO 488 100 ug $383
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BHA11901195-5 SMC-177D-A594 ATTO 594 100 ug $383
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BHA11901195-6 SMC-177D-ALP Alkaline Phosphatase 100 ug $376
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BHA11901195-7 SMC-177D-APC APC 100 ug $382
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BHA11901195-8 SMC-177D-BI Biotin 100 ug $378
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BHA11901195-9 SMC-177D-FITC FITC 100 ug $374
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BHA11901195-10 SMC-177D-HRP HRP 100 ug $370
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BHA11901195-11 SMC-177D-PCP PerCP 100 ug $382
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BHA11901195-12 SMC-177D-RPE RPE 100 ug $380
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BHA11901195-13 SMC-177S Unconjugated 12 ug $44
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