This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Clonality
polyclonal
Host
Rabbit
Immunogen
phosphatase and tensin homolog
Immunogen Region
phosphatase and tensin homolog
Isotype
IgG
Molecular Weight
53 kDa
Reactivity
Human, Mouse, Rat, Monkey
Recommended Dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Synonyms
MMAC1, TEP1
Uniprot
P60484
Gene Id
5728
Research Area
Cell Division and Proliferation, Signal Transduction, Metabolism, Cardiovascular, Immunology, Cancer, Stem Cells, Neuroscience
Form
liquid
Purification
Immunogen affinity purified
Purity
>=95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Application
WB: 1:500 - 1:2000; IHC: 1:50 - 1:100
Product Manual
<a href="https://www.fn-test.com/content/uploads/product/manuals/antibody/FNab09821.pdf" target="_blank">Data Sheet</ a>