Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by PIK3CG (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma) is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for PIK3CG.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, Liquid solution
Host
Rabbit
Immunogen
Synthesized peptide derived from human PI 3-kinase p85/p55 around the phosphorylation site of Y467/199
Immunogen Region
Synthesized peptide derived from human PI 3-kinase p85/p55 around the phosphorylation site of Y467/199
Isotype
Rabbit IgG
Molecular Weight
55, 85 KD
Others
Phospho-PI 3-kinase p85/p55 (Y467/199) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85/p55 protein only when phosphorylated at Y467/199.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Accession Number
P27986/Q92569
Gene Id
5295, 5295
Concentration
1 mg/ml, 1 mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen, The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Storage Buffer
PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage Instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.