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Home / Primary Antibodies / Monoclonal Antibodies

Phosphotyrosine Antibody, Clone G104 - SMC-174

BHA11901136
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[email protected]
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Target Phosphotyrosine
Clone No G104
Research Area Cell Signaling, Post-translational Modifications, Phosphorylation
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Scientific Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6).
In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).
Shipping Temperature Blue Ice or 4°C
Specificity Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP
Dilution WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Garton A.J., Flint A.J., and Tonks N.K. (1996) Mol and Cell Bio 16(11): 6408-6418.

2. Garton A.J., and Tonks N.K. (1999) J Bio Chem. 274(6): 3811-3818.

3. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.

4. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.

5. Downward J. (2001) Nature 411: 759-762.

6. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.

7. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352.

8. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656.

9. Tiganis T., Kemp B.E., and Tonks N.K. (1999) J. Bio Chem. 274(39): 27768-27775.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11901136-1 SMC-174D Unconjugated 100 ug $318
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BHA11901136-2 SMC-174D-A390 ATTO 390 100 ug $372
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BHA11901136-3 SMC-174D-A488 ATTO 488 100 ug $371
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BHA11901136-4 SMC-174D-A594 ATTO 594 100 ug $371
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BHA11901136-5 SMC-174D-ALP Alkaline Phosphatase 100 ug $363
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BHA11901136-6 SMC-174D-APC APC 100 ug $370
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BHA11901136-7 SMC-174D-BI Biotin 100 ug $366
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BHA11901136-8 SMC-174D-FITC FITC 100 ug $361
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BHA11901136-9 SMC-174D-HRP HRP 100 ug $356
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BHA11901136-10 SMC-174D-PCP PerCP 100 ug $370
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BHA11901136-11 SMC-174D-RPE RPE 100 ug $367
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BHA11901136-12 SMC-174S Unconjugated 12 ug $44
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