Phospho-ZO-2 (Tyr1118) Antibody

Mfr.No.	AF3874
Clonality	Polyclonal
Host	Rabbit
Immunogen	A synthesized peptide derived from human ZO-2 around the phosphorylation site of Tyr1118., A synthesized peptide derived from human ZO-2 around the phosphorylation site of Tyr1118.
Isotype	IgG
Reactivity	Human, Mouse, Rat

Rgd	AB_2847188
Uniprot	Q9UDY2
Gene Id	9414
Gene Name	TJP2

Storage	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	WB, IHC, ELISA
Dilution	IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Product Manual	Data Sheet

Western blot analysis of extracts from various samples, using Phospho-ZO-2 (Tyr1118) Antibody. Lane 1: Hela cells(LPS treatment), blocked with antigen-specific peptides, Lane 2: Hela cells(LPS treatment), Lane 3: Hela cells(heat shock treatment).Western blot analysis of extracts from various samples, using Phospho-ZO-2 (Tyr1118) Antibody.

AF3874 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3874 at 1/100 staining Human kidney cancer by IHC-P.

AF3874 at 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3874 at 1/100 staining Human ovarian cancer by IHC-P.

AF3874 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3874 at 1/100 staining Mouse colorectal tissue by IHC-P.

AF3874 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3874 at 1/100 staining Rat spleen tissue by IHC-P.