Phospho-Tuberin/TSC2 (Thr1462) Antibody

Mfr.No.	AF3334
Alternative Names	FLJ43106 ; LAM ; OTTHUMP00000158940 ; OTTHUMP00000198394 ; OTTHUMP00000198395 ; PPP1R160 ; Protein phosphatase 1, regulatory subunit 160 ; tsc2 ; TSC2_HUMAN ; TSC4 ; TSC4 gene, formerly ; TSC4, formerly ; Tuberin ; Tuberous sclerosis 2 ; Tuberous sclerosis 2 protein ; Tuberous sclerosis 2 protein homolog ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin.
Immunogen	A synthesized peptide derived from human Tuberin/TSC2 around the phosphorylation site of Threonine 1462.
Molecular Weight	Observed Mol.Wt.: 200 kDa. Predicted Mol.Wt.: 201kDa.
Reactivity	Human, Mouse, Rat
Specificity	Phospho-Tuberin/TSC2 (Thr1462) Antibody detects endogenous levels of Tuberin/TSC2 only when phosphorylated at Threonine 1462.
Subcellular Location	Cytoplasm. Membrane. At steady state found in association with membranes.

Uniprot

P49815

Gene Id

7249

Sequence

10	20	30	40	50
MAKPTSKDSG	LKEKFKILLG	LGTPRPNPRS	AEGKQTEFII	TAEILRELSM
60	70	80	90	100
ECGLNNRIRM	IGQICEVAKT	KKFEEHAVEA	LWKAVADLLQ	PERPLEARHA
110	120	130	140	150
VLALLKAIVQ	GQGERLGVLR	ALFFKVIKDY	PSNEDLHERL	EVFKALTDNG
160	170	180	190	200
RHITYLEEEL	ADFVLQWMDV	GLSSEFLLVL	VNLVKFNSCY	LDEYIARMVQ
210	220	230	240	250
MICLLCVRTA	SSVDIEVSLQ	VLDAVVCYNC	LPAESLPLFI	VTLCRTINVK
260	270	280	290	300
ELCEPCWKLM	RNLLGTHLGH	SAIYNMCHLM	EDRAYMEDAP	LLRGAVFFVG
310	320	330	340	350
MALWGAHRLY	SLRNSPTSVL	PSFYQAMACP	NEVVSYEIVL	SITRLIKKYR
360	370	380	390	400
KELQVVAWDI	LLNIIERLLQ	QLQTLDSPEL	RTIVHDLLTT	VEELCDQNEF
410	420	430	440	450
HGSQERYFEL	VERCADQRPE	SSLLNLISYR	AQSIHPAKDG	WIQNLQALME
460	470	480	490	500
RFFRSESRGA	VRIKVLDVLS	FVLLINRQFY	EEELINSVVI	SQLSHIPEDK
510	520	530	540	550
DHQVRKLATQ	LLVDLAEGCH	THHFNSLLDI	IEKVMARSLS	PPPELEERDV
560	570	580	590	600
AAYSASLEDV	KTAVLGLLVI	LQTKLYTLPA	SHATRVYEML	VSHIQLHYKH
610	620	630	640	650
SYTLPIASSI	RLQAFDFLLL	LRADSLHRLG	LPNKDGVVRF	SPYCVCDYME
660	670	680	690	700
PERGSEKKTS	GPLSPPTGPP	GPAPAGPAVR	LGSVPYSLLF	RVLLQCLKQE
710	720	730	740	750
SDWKVLKLVL	GRLPESLRYK	VLIFTSPCSV	DQLCSALCSM	LSGPKTLERL
760	770	780	790	800
RGAPEGFSRT	DLHLAVVPVL	TALISYHNYL	DKTKQREMVY	CLEQGLIHRC
810	820	830	840	850
ASQCVVALSI	CSVEMPDIII	KALPVLVVKL	THISATASMA	VPLLEFLSTL
860	870	880	890	900
ARLPHLYRNF	AAEQYASVFA	ISLPYTNPSK	FNQYIVCLAH	HVIAMWFIRC
910	920	930	940	950
RLPFRKDFVP	FITKGLRSNV	LLSFDDTPEK	DSFRARSTSL	NERPKSLRIA
960	970	980	990	1000
RPPKQGLNNS	PPVKEFKESS	AAEAFRCRSI	SVSEHVVRSR	IQTSLTSASL
1010	1020	1030	1040	1050
GSADENSVAQ	ADDSLKNLHL	ELTETCLDMM	ARYVFSNFTA	VPKRSPVGEF
1060	1070	1080	1090	1100
LLAGGRTKTW	LVGNKLVTVT	TSVGTGTRSL	LGLDSGELQS	GPESSSSPGV
1110	1120	1130	1140	1150
HVRQTKEAPA	KLESQAGQQV	SRGARDRVRS	MSGGHGLRVG	ALDVPASQFL
1160	1170	1180	1190	1200
GSATSPGPRT	APAAKPEKAS	AGTRVPVQEK	TNLAAYVPLL	TQGWAEILVR
1210	1220	1230	1240	1250
RPTGNTSWLM	SLENPLSPFS	SDINNMPLQE	LSNALMAAER	FKEHRDTALY
1260	1270	1280	1290	1300
KSLSVPAAST	AKPPPLPRSN	TVASFSSLYQ	SSCQGQLHRS	VSWADSAVVM
1310	1320	1330	1340	1350
EEGSPGEVPV	LVEPPGLEDV	EAALGMDRRT	DAYSRSSSVS	SQEEKSLHAE
1360	1370	1380	1390	1400
ELVGRGIPIE	RVVSSEGGRP	SVDLSFQPSQ	PLSKSSSSPE	LQTLQDILGD
1410	1420	1430	1440	1450
PGDKADVGRL	SPEVKARSQS	GTLDGESAAW	SASGEDSRGQ	PEGPLPSSSP
1460	1470	1480	1490	1500
RSPSGLRPRG	YTISDSAPSR	RGKRVERDAL	KSRATASNAE	KVPGINPSFV
1510	1520	1530	1540	1550
FLQLYHSPFF	GDESNKPILL	PNESQSFERS	VQLLDQIPSY	DTHKIAVLYV
1560	1570	1580	1590	1600
GEGQSNSELA	ILSNEHGSYR	YTEFLTGLGR	LIELKDCQPD	KVYLGGLDVC
1610	1620	1630	1640	1650
GEDGQFTYCW	HDDIMQAVFH	IATLMPTKDV	DKHRCDKKRH	LGNDFVSIVY
1660	1670	1680	1690	1700
NDSGEDFKLG	TIKGQFNFVH	VIVTPLDYEC	NLVSLQCRKD	MEGLVDTSVA
1710	1720	1730	1740	1750
KIVSDRNLPF	VARQMALHAN	MASQVHHSRS	NPTDIYPSKW	IARLRHIKRL
1760	1770	1780	1790	1800
RQRICEEAAY	SNPSLPLVHP	PSHSKAPAQT	PAEPTPGYEV	GQRKRLISSV

EDFTEFV

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.