Phospho-TFEB (Ser211) Antibody

Mfr.No.	AF3708
Clonality	Polyclonal
Host	Rabbit
Immunogen	A synthesized peptide derived from human TFEB around the phosphorylation site of Ser211., A synthesized peptide derived from human TFEB around the phosphorylation site of Ser211.
Isotype	IgG
Reactivity	Human, Mouse, Rat

Rgd	AB_2847022
Uniprot	P19484
Gene Id	7942
Gene Name	TFEB

Storage	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	WB, IHC, IF/ICC, ELISA
Dilution	WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Product Manual	Data Sheet

AF3708 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3708 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

AF3708 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3708 at 1/100 staining Human kidney cancer by IHC-P.

AF3708 staining A549 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF3708) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).AF3708 staining A549 cells(4h of LPS treatment) by IF/ICC.

Fig. 7 FGF21 boosted autophagy via enhancing TFEB activity. On the 7th day after operation, samples were harvested from the Control andFGF21 groups to evaluate the TFEB level. Further, transfection of the AVV TFEB shRNA was performed to inhibit the expression of TFEB in the cells,and then the expression of relative proteins was compared in the FGF21, Scramble and TFEB shRNA group. a Immunofluorescence exhibiting moreexpression of TFEB in the FGF21 group than that in the Control group (scan bar, 15 ?m). b Histogram showing the percentage of TFEB translocationinto nucleus in dermal layer. c Western blotting showing levels of P-TFEB (Ser221) and nuclear TFEB. d Histogram showing quantificationalcomparison of cytoplasmic and nuclear TFEB expressions between the Control and FGF21 groups. e Immunofluorescence exhibiting the expressionof LC3II in the FGF21, Scramble and TFEB shRNA groups. f Quantification of percentage of positive cells with LC3II labeled autophagosomes in dermallayer. g West

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SKU	Supplier No.	Size	Your price	Quantity
BHA12913458	AF3708	100ul	$350		Add to Cart
BHA12913458	AF3708	200ul	$450		Add to Cart

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Phospho-TFEB (Ser211) Antibody

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