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Home / Primary Antibodies

Phospho-Smad1/9 (Ser463/Ser465) Antibody

SKU : BHA12906279

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	AF8454
Alternative Names	BSP-1 ; BSP1 ; HsMAD1 ; JV4-1 ; JV41 ; MAD homolog 1 ; MAD mothers against decapentaplegic homolog 1 ; Mad related protein 1 ; Mad-related protein 1 ; MADH1 ; MADR1 ; Mothers against decapentaplegic homolog 1 ; Mothers against DPP homolog 1 ; SMA- AND MAD-RELATED PROTEIN 1 ; SMAD 1 ; SMAD family member 1 ; SMAD mothers against DPP homolog 1 ; Smad1 ; SMAD1_HUMAN ; TGF beta signaling protein 1 ; Transforming growth factor-beta-signaling protein 1 ; MAD homolog 9 ; Madh6 ; Mothers against decapentaplegic ; Mothers against decapentaplegic homolog 9 ; Mothers against DPP homolog 9 ; SMAD 9 ; SMAD family member 9 ; SMAD, mothers against DPP homolog 9 (Drosophila) ; SMAD8A ; SMAD8B ; Smad9 ; SMAD9_HUMAN ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Immunogen	A synthesized peptide derived from human Smad1/9 (Phospho-Ser463/Ser465).
Molecular Weight	Observed Mol.Wt.: 52KD. Predicted Mol.Wt.: 53kDa.
Reactivity	Human, Mouse, Rat
Specificity	Smad1/9 (Phospho-Ser463/Ser465) Antibody detects endogenous levels of Smad1/9 only when phosphorylated at Ser463/Ser465.
Subcellular Location	Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane.

Uniprot

Q15797 ; O15198

Gene Id

40864093

Sequence

10	20	30	40	50
MNVTSLFSFT	SPAVKRLLGW	KQGDEEEKWA	EKAVDALVKK	LKKKKGAMEE
60	70	80	90	100
LEKALSCPGQ	PSNCVTIPRS	LDGRLQVSHR	KGLPHVIYCR	VWRWPDLQSH
110	120	130	140	150
HELKPLECCE	FPFGSKQKEV	CINPYHYKRV	ESPVLPPVLV	PRHSEYNPQH
160	170	180	190	200
SLLAQFRNLG	QNEPHMPLNA	TFPDSFQQPN	SHPFPHSPNS	SYPNSPGSSS
210	220	230	240	250
STYPHSPTSS	DPGSPFQMPA	DTPPPAYLPP	EDPMTQDGSQ	PMDTNMMAPP
260	270	280	290	300
LPSEINRGDV	QAVAYEEPKH	WCSIVYYELN	NRVGEAFHAS	STSVLVDGFT
310	320	330	340	350
DPSNNKNRFC	LGLLSNVNRN	STIENTRRHI	GKGVHLYYVG	GEVYAECLSD
360	370	380	390	400
SSIFVQSRNC	NYHHGFHPTT	VCKIPSGCSL	KIFNNQEFAQ	LLAQSVNHGF
410	420	430	440	450
ETVYELTKMC	TIRMSFVKGW	GAEYHRQDVT	STPCWIEIHL	HGPLQWLDKV
460
LTQMGSPHNP	ISSVS

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	ELISA, WB
Dilution	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
Sample Preparation For Western Blotting.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12906279	AF8454	100ul	$350		Add to Cart
BHA12906279	AF8454	200ul	$450		Add to Cart

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