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Home / Antibodies

Phospho-RB1 (S249) Antibody

BHA10540923
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Tel
+1 866.986.9598
Email
[email protected]
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Uniprot P06400
Database Links

HGNC:9884

OMIM:109800

KEGG:hsa:5925

STRING:9606.ENSP00000267163

UniGene:Hs.408528
Function Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Pathway Cell cycle Cellular senescence
Protein Families Retinoblastoma protein (RB) family
Tissue Specificity Expressed in the retina.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Format Liquid
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications IF, ELISA
Description IF ; ELISA
Dilution

IF1:200-1:1000
ELISA1/5000
Tested Application IF,ELISA;IF:1/200-1/1000.ELISA:1/5000
Datasheet
Immunofluorescence (IF) Protocol
ELISA Protocol
SKU Supplier No. Size Your price Quantity
BHA10540923 CSB-PA040171 100μg $168.78
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BHA10540923 CSB-PA040171 50μg $123
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