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Home / Antibodies

Phospho-PDPK1 (Tyr376) Antibody

SKU : BHA12904920

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	AF8123
Alternative Names	3 phosphoinositide dependent protein kinase 1 ; 3-phosphoinositide-dependent protein kinase 1 ; hPDK 1 ; hPDK1 ; MGC20087 ; MGC35290 ; OTTHUMP00000159109 ; OTTHUMP00000159110 ; OTTHUMP00000174525 ; PDK1 ; Pdpk1 ; PDPK1_HUMAN ; PDPK2 ; PDPK2P ; PkB kinase ; PkB kinase like gene 1 ; PkB like 1 ; PRO0461 ; Protein kinase ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Immunogen	A synthesized peptide derived from human PDK1 (Phospho-Tyr376).
Molecular Weight	Observed Mol.Wt.: 63kDa. Predicted Mol.Wt.: 64kDa.
Reactivity	Human, Mouse, Rat
Specificity	PDK1 (Phospho-Tyr376) Antibody detects endogenous levels of PDK1 only when phosphorylated at Tyr376.
Subcellular Location	Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.

Uniprot

O15530

Gene Id

5170

Sequence

10	20	30	40	50
MARTTSQLYD	AVPIQSSVVL	CSCPSPSMVR	TQTESSTPPG	IPGGSRQGPA
60	70	80	90	100
MDGTAAEPRP	GAGSLQHAQP	PPQPRKKRPE	DFKFGKILGE	GSFSTVVLAR
110	120	130	140	150
ELATSREYAI	KILEKRHIIK	ENKVPYVTRE	RDVMSRLDHP	FFVKLYFTFQ
160	170	180	190	200
DDEKLYFGLS	YAKNGELLKY	IRKIGSFDET	CTRFYTAEIV	SALEYLHGKG
210	220	230	240	250
IIHRDLKPEN	ILLNEDMHIQ	ITDFGTAKVL	SPESKQARAN	SFVGTAQYVS
260	270	280	290	300
PELLTEKSAC	KSSDLWALGC	IIYQLVAGLP	PFRAGNEYLI	FQKIIKLEYD
310	320	330	340	350
FPEKFFPKAR	DLVEKLLVLD	ATKRLGCEEM	EGYGPLKAHP	FFESVTWENL
360	370	380	390	400
HQQTPPKLTA	YLPAMSEDDE	DCYGNYDNLL	SQFGCMQVSS	SSSSHSLSAS
410	420	430	440	450
DTGLPQRSGS	NIEQYIHDLD	SNSFELDLQF	SEDEKRLLLE	KQAGGNPWHQ
460	470	480	490	500
FVENNLILKM	GPVDKRKGLF	ARRRQLLLTE	GPHLYYVDPV	NKVLKGEIPW
510	520	530	540	550
SQELRPEAKN	FKTFFVHTPN	RTYYLMDPSG	NAHKWCRKIQ	EVWRQRYQSH

PDAAVQ

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	ELISA, IHC, WB
Dilution	WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12904920	AF8123	100ul	$350		Add to Cart
BHA12904920	AF8123	200ul	$450		Add to Cart

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