Phospho-PDPK1 (Thr245) Antibody

Mfr.No.	AF7115
Alternative Names	3 phosphoinositide dependent protein kinase 1 ; 3-phosphoinositide-dependent protein kinase 1 ; hPDK 1 ; hPDK1 ; MGC20087 ; MGC35290 ; OTTHUMP00000159109 ; OTTHUMP00000159110 ; OTTHUMP00000174525 ; PDK1 ; Pdpk1 ; PDPK1_HUMAN ; PDPK2 ; PDPK2P ; PkB kinase ; PkB kinase like gene 1 ; PkB like 1 ; PRO0461 ; Protein kinase ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Immunogen	A synthesized peptide derived from human PDK1 around the phosphorylation site of Thr245.
Molecular Weight	Observed Mol.Wt.: 63kDa. Predicted Mol.Wt.: 64kDa.
Reactivity	Human, Mouse, Rat
Specificity	Phospho-PDK1 (Thr245) Antibody detects endogenous levels of PDK1 only when phosphorylated at Thr245.
Subcellular Location	Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.

Uniprot

O15530

Gene Id

5170

Sequence

10 20 30 40 50
MARTTSQLYD AVPIQSSVVL CSCPSPSMVR TQTESSTPPG IPGGSRQGPA
60 70 80 90 100
MDGTAAEPRP GAGSLQHAQP PPQPRKKRPE DFKFGKILGE GSFSTVVLAR
110 120 130 140 150
ELATSREYAI KILEKRHIIK ENKVPYVTRE RDVMSRLDHP FFVKLYFTFQ
160 170 180 190 200
DDEKLYFGLS YAKNGELLKY IRKIGSFDET CTRFYTAEIV SALEYLHGKG
210 220 230 240 250
IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN SFVGTAQYVS
260 270 280 290 300
PELLTEKSAC KSSDLWALGC IIYQLVAGLP PFRAGNEYLI FQKIIKLEYD
310 320 330 340 350
FPEKFFPKAR DLVEKLLVLD ATKRLGCEEM EGYGPLKAHP FFESVTWENL
360 370 380 390 400
HQQTPPKLTA YLPAMSEDDE DCYGNYDNLL SQFGCMQVSS SSSSHSLSAS
410 420 430 440 450
DTGLPQRSGS NIEQYIHDLD SNSFELDLQF SEDEKRLLLE KQAGGNPWHQ
460 470 480 490 500
FVENNLILKM GPVDKRKGLF ARRRQLLLTE GPHLYYVDPV NKVLKGEIPW
510 520 530 540 550
SQELRPEAKN FKTFFVHTPN RTYYLMDPSG NAHKWCRKIQ EVWRQRYQSH

PDAAVQ

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.