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Home / Primary Antibodies

Phospho-NCF1/p47-phox (Ser359) Antibody

SKU : BHA12901566

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	AF3167
Alternative Names	47 kDa autosomal chronic granulomatous disease protein ; 47 kDa neutrophil oxidase factor ; NADPH oxidase organizer 2 ; NCF 47K ; NCF-1 ; NCF-47K ; Ncf1 ; NCF1_HUMAN ; Neutrophil cytosol factor 1 ; Neutrophil cytosolic factor 1 ; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) ; Neutrophil NADPH oxidase factor 1 ; Nox organizer 2 ; Nox organizing protein 2 ; Nox-organizing protein 2 ; NOXO2 ; p47 phox ; p47-phox ; SH3 and PX domain containing protein 1A ; SH3 and PX domain-containing protein 1A ; SH3PXD1A ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	The 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase is found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains.
Immunogen	A synthesized peptide derived from human p47 phox around the phosphorylation site of Serine 359.
Molecular Weight	Observed Mol.Wt.: 45kDa. Predicted Mol.Wt.: 45kDa.
Reactivity	Human, Rat
Specificity	Phospho-p47 phox (Ser359) Antibody detects endogenous levels of p47 phox only when phosphorylated at Serine 359.
Subcellular Location	Cytoplasm.

Uniprot

P14598

Gene Id

653361

Sequence

10	20	30	40	50
MGDTFIRHIA	LLGFEKRFVP	SQHYVYMFLV	KWQDLSEKVV	YRRFTEIYEF
60	70	80	90	100
HKTLKEMFPI	EAGAINPENR	IIPHLPAPKW	FDGQRAAENR	QGTLTEYCST
110	120	130	140	150
LMSLPTKISR	CPHLLDFFKV	RPDDLKLPTD	NQTKKPETYL	MPKDGKSTAT
160	170	180	190	200
DITGPIILQT	YRAIANYEKT	SGSEMALSTG	DVVEVVEKSE	SGWWFCQMKA
210	220	230	240	250
KRGWIPASFL	EPLDSPDETE	DPEPNYAGEP	YVAIKAYTAV	EGDEVSLLEG
260	270	280	290	300
EAVEVIHKLL	DGWWVIRKDD	VTGYFPSMYL	QKSGQDVSQA	QRQIKRGAPP
310	320	330	340	350
RRSSIRNAHS	IHQRSRKRLS	QDAYRRNSVR	FLQQRRRQAR	PGPQSPGSPL
360	370	380	390
EEERQTQRSK	PQPAVPPRPS	ADLILNRCSE	STKRKLASAV

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	IF/ICC, ELISA, IHC, WB
Dilution	WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12901566	AF3167	100ul	$280		Add to Cart
BHA12901566	AF3167	200ul	$350		Add to Cart

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