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Home / Primary Antibodies

Phospho-IKB alpha (Ser32/Ser36) Antibody

SKU : BHA12901646

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	AF2002
Alternative Names	I kappa B alpha ; I-kappa-B-alpha ; IkappaBalpha ; IkB-alpha ; IKBA ; IKBA_HUMAN ; IKBalpha ; MAD 3 ; MAD3 ; Major histocompatibility complex enhancer-binding protein MAD3 ; NF kappa B inhibitor alpha ; NF-kappa-B inhibitor alpha ; NFKBI ; NFKBIA ; Nuclear factor of kappa light chain gene enhancer in B cells ; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm.
Immunogen	A synthesized peptide derived from human I kappaB- alpha around the phosphorylation site of Serine 32/Serine 36.
Molecular Weight	Observed Mol.Wt.: 39kDa. Predicted Mol.Wt.: 36kDa.
Reactivity	Human, Mouse, Rat
Specificity	Phospho-I kappaB- alpha (Ser32/Ser36) Antibody detects endogenous levels of I kappaB- alpha only when phosphorylated at Serine 32/Serine 36.
Subcellular Location	Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

Uniprot

P25963

Gene Id

4792

Sequence

10	20	30	40	50
MFQAAERPQE	WAMEGPRDGL	KKERLLDDRH	DSGLDSMKDE	EYEQMVKELQ
60	70	80	90	100
EIRLEPQEVP	RGSEPWKQQL	TEDGDSFLHL	AIIHEEKALT	MEVIRQVKGD
110	120	130	140	150
LAFLNFQNNL	QQTPLHLAVI	TNQPEIAEAL	LGAGCDPELR	DFRGNTPLHL
160	170	180	190	200
ACEQGCLASV	GVLTQSCTTP	HLHSILKATN	YNGHTCLHLA	SIHGYLGIVE
210	220	230	240	250
LLVSLGADVN	AQEPCNGRTA	LHLAVDLQNP	DLVSLLLKCG	ADVNRVTYQG
260	270	280	290	300
YSPYQLTWGR	PSTRIQQQLG	QLTLENLQML	PESEDEESYD	TESEFTEFTE
310
DELPYDDCVF	GGQRLTL

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	IF/ICC, ELISA, IHC, WB
Dilution	WB 1:500-1:2000 IHC 1:50-1:500 IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12901646	AF2002	100ul	$280		Add to Cart
BHA12901646	AF2002	200ul	$350		Add to Cart

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