Phospho-IFNAR1 (Tyr466) Antibody

Mfr.No.	AF3717
Clonality	Polyclonal
Host	Rabbit
Immunogen	A synthesized peptide derived from human IFNAR1 around the phosphorylation site of Tyr466., A synthesized peptide derived from human IFNAR1 around the phosphorylation site of Tyr466.
Isotype	IgG
Reactivity	Human, Mouse, Rat

Rgd	AB_2847031
Uniprot	P17181
Gene Id	3454
Gene Name	IFNAR1

Storage	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	IHC, IF/ICC, ELISA
Dilution	IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Product Manual	Data Sheet

AF3717 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3717 at 1/100 staining Mouse testis tissue by IHC-P.

AF3717 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3717 at 1/100 staining Rat kidney tissue by IHC-P.

AF3717 staining Hela cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF3717) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).AF3717 staining Hela cells(heat shock treatment) by IF/ICC.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12913467	AF3717	100ul	$350		Add to Cart
BHA12913467	AF3717	200ul	$450		Add to Cart

Phospho-IFNAR1 (Tyr466) Antibody

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