Phospho-GSK3 beta (Thr390) Antibody

Mfr.No.	AF3658
Clonality	Polyclonal
Host	Rabbit
Immunogen	A synthesized peptide derived from human GSK3 beta around the phosphorylation site of Thr390., A synthesized peptide derived from human GSK3 beta around the phosphorylation site of Thr390.
Isotype	IgG
Reactivity	Human, Mouse, Rat

Rgd	AB_2846972
Uniprot	P49841
Gene Id	2932
Gene Name	GSK3B

Storage	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	WB, IHC, IF/ICC, ELISA
Dilution	WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Product Manual	Data Sheet

Western blot analysis of extracts from HeLa cells, cells treated with hydroxyurea, using Phospho-GSK3B (Thr390) Antibody . The lane on the left was treated with blocking peptide.Western blot analysis of extracts from HeLa cells, cells treated with hydroxyurea, using Phospho-GSK3B (Thr390) Antibody .

AF3658 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3658 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

AF3658 at 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3658 at 1/100 staining Human kidney cancer by IHC-P.

AF3658 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3658 at 1/100 staining Mouse kidney tissue by IHC-P.

AF3658 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3658 at 1/100 staining Mouse brain tissue by IHC-P.

AF3658 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.AF3658 at 1/100 staining Mouse colorectal tissue by IHC-P.