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Home / Primary Antibodies

Phospho-ERK1/2 (Thr202/Tyr204) Antibody

SKU : BHA12900766

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	AF1015
Alternative Names	ERK 1 ; ERK ; ERK-1 ; ERK1 ; ERT 2 ; ERT2 ; Extracellular Signal Regulated Kinase 1 ; Extracellular signal related kinase 1 ; Extracellular signal-regulated kinase 1 ; HGNC6877 ; HS44KDAP ; HUMKER1A ; Insulin Stimulated MAP2 Kinase ; Insulin-stimulated MAP2 kinase ; MAP kinase 1 ; MAP kinase 3 ; MAP Kinase ; MAP kinase isoform p44 ; MAPK 1 ; MAPK 3 ; MAPK ; MAPK1 ; Mapk3 ; MGC20180 ; Microtubule Associated Protein 2 Kinase ; Microtubule-associated protein 2 kinase ; Mitogen Activated Protein Kinase 3 ; Mitogen-activated protein kinase 1 ; Mitogen-activated protein kinase 3 ; MK03_HUMAN ; OTTHUMP00000174538 ; OTTHUMP00000174541 ; p44 ERK1 ; p44 MAPK ; p44-ERK1 ; p44-MAPK ; P44ERK1 ; P44MAPK ; PRKM 3 ; PRKM3 ; Protein Kinase Mitogen Activated 3 ; ERK 2 ; ERK ; ERK-2 ; ERT1 ; Extracellular Signal Regulated Kinase 2 ; Extracellular signal-regulated kinase 2 ; MAP kinase 1 ; MAP kinase 2 ; MAP kinase isoform p42 ; MAPK 1 ; MAPK 2 ; Mapk1 ; MAPK2 ; Mitogen-activated protein kinase 1 ; Mitogen-activated protein kinase 2 ; MK01_HUMAN ; P38 ; P40 ; P41 ; p42-MAPK ; P42MAPK ; PRKM1 ; PRKM2 ; protein kinase, mitogen-activated, 1 ; protein kinase, mitogen-activated, 2 ; protein tyrosine kinase ERK2 ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.Publishing reference https://www.spandidos-publications.com/10.3892/mmr.2016.5305/downloadhttp://www.sciencedirect.com/science/article/pii/S0306452216000464http://www.sciencedirect.com/science/article/pii/S0171298514002782https://www.spandidos-publications.com/etm/12/1/499/download
Immunogen	A synthesized peptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204.
Molecular Weight	Observed Mol.Wt.: 42,44kDa. Predicted Mol.Wt.: 44kDa.
Reactivity	Human, Mouse, Rat
Specificity	Phospho-ERK1/2 (Thr202/Tyr204) Antibody detects endogenous levels of ERK1/2 only when phosphorylated at Threonine 202/Tyrosine 204.
Subcellular Location	Nucleus.

Uniprot

P27361 ; P28482

Gene Id

55955594

Sequence

10	20	30	40	50
MAAAAAQGGG	GGEPRRTEGV	GPGVPGEVEM	VKGQPFDVGP	RYTQLQYIGE
60	70	80	90	100
GAYGMVSSAY	DHVRKTRVAI	KKISPFEHQT	YCQRTLREIQ	ILLRFRHENV
110	120	130	140	150
IGIRDILRAS	TLEAMRDVYI	VQDLMETDLY	KLLKSQQLSN	DHICYFLYQI
160	170	180	190	200
LRGLKYIHSA	NVLHRDLKPS	NLLINTTCDL	KICDFGLARI	ADPEHDHTGF
210	220	230	240	250
LTEYVATRWY	RAPEIMLNSK	GYTKSIDIWS	VGCILAEMLS	NRPIFPGKHY
260	270	280	290	300
LDQLNHILGI	LGSPSQEDLN	CIINMKARNY	LQSLPSKTKV	AWAKLFPKSD
310	320	330	340	350
SKALDLLDRM	LTFNPNKRIT	VEEALAHPYL	EQYYDPTDEP	VAEEPFTFAM
360	370
ELDDLPKERL	KELIFQETAR	FQPGVLEAP

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	IF/ICC, ELISA, IHC, WB
Dilution	WB 1:500-1:2000 IHC 1:50-1:200 IF 1:200, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IHC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12900766	AF1015	100ul	$280		Add to Cart
BHA12900766	AF1015	200ul	$350		Add to Cart

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