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Home / Antibodies

Phospho-CHEK1 (S286) Antibody

BHA10539414
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Email
[email protected]
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Uniprot O14757
Database Links

HGNC:1925

OMIM:603078

KEGG:hsa:1111

STRING:9606.ENSP00000388648

UniGene:Hs.24529
Function Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.; FUNCTION
Pathway p53 signaling pathway Cell cycle Cellular senescence
Protein Families Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily
Tissue Specificity Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Format Liquid
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications WB, IF, ELISA
Description WB ; IF ; ELISA, WB ; ELISA ; IF
Dilution

WB1:500-1:2000
IF1:200-1:1000
ELISA1/5000
Tested Application WB,IF,ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000
Datasheet
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
SKU Supplier No. Size Your price Quantity
BHA10539414 CSB-PA006770 100μg $168.78
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BHA10539414 CSB-PA006770 50μg $123
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