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Home / Antibodies

Phospho-AKT1 (Ser473+Tyr474) Antibody

SKU : BHA12904769

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	AF8357
Alternative Names	AKT 1 ; AKT ; AKT1 ; AKT1_HUMAN ; MGC99656 ; PKB ; PKB-ALPHA ; PRKBA ; Protein Kinase B Alpha ; Protein kinase B ; Proto-oncogene c-Akt ; RAC Alpha ; RAC ; RAC-alpha serine/threonine-protein kinase ; RAC-PK-alpha ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Immunogen	A synthesized peptide derived from human AKT1 (Phospho-Ser473+Tyr474).
Molecular Weight	Observed Mol.Wt.: 60kDa. Predicted Mol.Wt.: 56kDa.
Reactivity	Human
Specificity	AKT1 (Phospho-Ser473+Tyr474) Antibody detects endogenous levels of AKT1 only when phosphorylated at Ser473+Tyr474.
Subcellular Location	Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

Uniprot

P31749

Gene Id

207

Sequence

10	20	30	40	50
MSDVAIVKEG	WLHKRGEYIK	TWRPRYFLLK	NDGTFIGYKE	RPQDVDQREA
60	70	80	90	100
PLNNFSVAQC	QLMKTERPRP	NTFIIRCLQW	TTVIERTFHV	ETPEEREEWT
110	120	130	140	150
TAIQTVADGL	KKQEEEEMDF	RSGSPSDNSG	AEEMEVSLAK	PKHRVTMNEF
160	170	180	190	200
EYLKLLGKGT	FGKVILVKEK	ATGRYYAMKI	LKKEVIVAKD	EVAHTLTENR
210	220	230	240	250
VLQNSRHPFL	TALKYSFQTH	DRLCFVMEYA	NGGELFFHLS	RERVFSEDRA
260	270	280	290	300
RFYGAEIVSA	LDYLHSEKNV	VYRDLKLENL	MLDKDGHIKI	TDFGLCKEGI
310	320	330	340	350
KDGATMKTFC	GTPEYLAPEV	LEDNDYGRAV	DWWGLGVVMY	EMMCGRLPFY
360	370	380	390	400
NQDHEKLFEL	ILMEEIRFPR	TLGPEAKSLL	SGLLKKDPKQ	RLGGGSEDAK
410	420	430	440	450
EIMQHRFFAG	IVWQHVYEKK	LSPPFKPQVT	SETDTRYFDE	EFTAQMITIT
460	470	480
PPDQDDSMEC	VDSERRPHFP	QFSYSASGTA

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	IF/ICC, ELISA, WB
Dilution	WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
IF/ICC Protocol.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12904769	AF8357	100ul	$350		Add to Cart
BHA12904769	AF8357	200ul	$450		Add to Cart

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