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Home / Primary Antibodies / Polyclonal Antibodies

PDI Antibody - SPC-114

BHA11906443
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+1 866.986.9598
Email
[email protected]
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Swissprot P04785
Accession Number NP_001099245.2
Gene Id 287164
Target PDI
Research Area Cell Signaling, Chaperone Proteins, Tags and Cell Markers, Organelle Markers, Protein Trafficking
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity Purified
Scientific Background The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5).
PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~58kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. NA K.S. et al. (2007) Mol Cells. 24(2): 261-7.

2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5.

3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42.

4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95.

5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163.

6. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819.

7. Yoshimori T., et al. (1990) J Biol Chem. 265(26): 15984-90.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906443-1 SPC-114D Unconjugated 100 ul $334
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BHA11906443-2 SPC-114C Unconjugated 25 ul $150
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BHA11906443-3 SPC-114D-A390 ATTO 390 100 ul $388
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BHA11906443-4 SPC-114D-A488 ATTO 488 100 ul $387
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BHA11906443-5 SPC-114D-A594 ATTO 594 100 ul $387
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BHA11906443-6 SPC-114D-ALP Alkaline Phosphatase 100 ul $381
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BHA11906443-7 SPC-114D-APC APC 100 ul $386
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BHA11906443-8 SPC-114D-BI Biotin 100 ul $383
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BHA11906443-9 SPC-114D-FITC FITC 100 ul $378
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BHA11906443-10 SPC-114D-HRP HRP 100 ul $374
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BHA11906443-11 SPC-114D-PCP PerCP 100 ul $386
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BHA11906443-12 SPC-114D-RPE RPE 100 ul $384
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BHA11906443-13 SPC-114S Unconjugated 12 ul $44
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